pGL4.12[luc2CP] Vector Protocol
Instructions for Use of Product(s)
Literature # 9PIE667
The pGL4.12[luc2CP] Vector encodes the luciferase reporter gene luc2CP (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been optimized for mammalian expression. The pGL4.12[luc2CP] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice. The luc2CP reporter gene contains hCL1 and hPEST, both of which are protein destabilization sequences. The protein encoded by luc2CP responds more quickly and with greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.
Printed in USA. Revised 10/16.