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pFN10A (ACT) Flexi® Vector

Instructions for Use of Product(s)

Literature # 9PIC933

The pFN10A (ACT) Flexi® Vector is designed for use with the CheckMate™ /Flexi® Vector Mammalian Two-Hybrid System (Cat.# C9360), the Flexi® System, Entry/Transfer (Cat.# C8640 )and the Flexi® System, Transfer (Cat.# C8820). The pFN10A (ACT) Flexi® Vector contains a herpes simplex virus VP16 activation domain upstream and in-frame of the cloning site. The pFN10A (ACT) Flexi® Vector also contains a neomycin resistance gene under the control of the SV40 promoter for long-term selection of transfected mammalian cells. Transfection of a pFN10A (ACT) Flexi® Vector containing a cloned protein coding region into mammalian cells provides resistance to the antibiotic G-418 (Cat.# V7981 or V8091) and allows selection of transfectants. In common with other Flexi® Vectors, the pFN10A (ACT) Flexi® Vector contains a CMV immediate early enhancer/promoter region plus a chimeric intron for mammalian expression, a T7 promoter for in vitro expression of the protein-coding region, a barnase gene for positive selection of the insert, an ampicillin resistance gene for selection of the plasmid, and unique Sgf I and Pme I sites that allow easy insertion and transfer of the insert of interest. Do not use the pFN10A (ACT) Flexi® Vector without an insert as a negative control because the presence of the barnase gene decreases the viability of the transfected cells. The pFN10A (ACT) Flexi® Vector containing a cloned protein-coding region can be used to transfer the insert to other Flexi® Vectors with different expression options using the Flexi® Systems. For more information, see the CheckMate™/Flexi® Vector Mammalian Two-Hybrid System Technical Manual #TM283 and the Flexi ® Vector Systems Technical Manual #TM254.

Printed in USA. Revised 10/16.