Instructions for Use of Product(s)
Literature # 9PIC936
The pF9A CMV hRluc-neo Flexi® Vector is designed for use with the Flexi® System, Entry/Transfer (Cat.#C8640) and the Flexi® System, Transfer (Cat.# C8820). The vector contains a CMV immediate early enhancer/promoter region plus a chimeric intron for mammalian expression, and a T7 promoter for in vitro expression of the protein-coding region. In addition, the vector contains a barnase gene for positive selection of the insert, an ampicillin-resistance gene for selection of the plasmid and unique SgfI and PmeI sites that allow easy insertion and transfer of the insert of interest. The pF9A CMV hRluc-neo Flexi® Vector also contains a unique neomycin-resistance gene fused with a humanized Renilla luciferase (hRluc) gene, under the control of the SV40 promoter, allowing long-term selection of transfected mammalian cells and the ability to monitor those selected cells using a Renilla luciferase assay. Transfection of the pF9A CMV hRluc-neo Flexi® Vector containing a protein-coding region into mammalian cells provides resistance to the antibiotic G-418 (Cat.# V7981 or V8091) and allows selection of transfectants.
Printed in USA. Revised 9/16