Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

pF5A CMV-neo Flexi® Vector Product Information

Instructions for Use of Product(s)

Literature # 9PIC940

The pF5A CMV-neo Flexi® Vector is designed for use with the Flexi® System, Entry/Transfer (Cat.# C8640) and the Flexi® System, Transfer (Cat.# C8820). The pF5A CMV-neo Flexi® Vector contains the CMV immediate early enhancer/promoter region plus a chimeric intron for mammalian expression and a T7 promoter for in vitro expression of the protein-coding region. The vector also contains the barnase gene for positive selection of the insert, an ampicillin-resistance gene for selection of the plasmid, and unique SgfI and PmeI sites that allow easy insertion and transfer of the insert of interest. The pF5A CMV-neo Flexi® Vector also contains a neomycin resistance gene under the control of the SV40 promoter for long-term selection of transfected mammalian cells. Transfection of a pF5A CMV-neo Flexi® Vector containing a cloned protein-coding region into mammalian cells provides resistance to the antibiotic G-418 (Cat.# V7981, V8091), allowing selection of transfectants.

Printed in USA. Revised 10/16.