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PolyATtract® mRNA Isolation Systems

Isolates Highly Pure mRNA from Total RNA

  • Protocol complete in approximately 45 minutes
  • Isolated mRNA is suitable for use in in vitro translation, RT-PCR and cDNA synthesis
  • Cat.# Z5200 and Z5210 contain sufficient reagents for 3 separate mRNA isolations, each from 1-5mg total RNA
  • Cat.# Z5300 and Z5310 contain sufficient reagents for 15 separate mRNA isolations, each from 100-1,000µg total RNA

Size

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Catalog number selected: Z5210

$ 195.00 Your price: Log In

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PolyATtract® mRNA Isolation Systems
3 isolations
$ 195.00
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The PolyATtract® mRNA Isolation Systems use the MagneSphere® technology to isolate mRNA rapidly and effectively from total RNA. The systems use a biotinylated oligo(dT) primer to hybridize, at high efficiency in solution, to the 3´ poly(A)+ region present in most mature eukaryotic mRNAs. The hybrids are bound to streptavidin coupled to paramagnetic particles, captured using a magnetic separation stand and washed at high stringency. The highly pure mRNA is eluted from the solid phase by the simple addition of ribonuclease-free, deionized water. With total RNA as the starting material, poly(A)+ mRNA is isolated in approximately 45 minutes. The isolated mRNA is suitable for all molecular biology applications, including in vitro translation and cDNA synthesis.

Protocols

Specifications

You are viewing: 3 isolations Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Nuclease-Free Water

P119C 3 x 25ml View Product

SSC, 20X

Z525A 3 x 1.4ml

Biotinylated Oligo(dT) Probe

Z526A 1 x 35μl View Product

mRNA User Tube

Z528A 3 x 1 each

Streptavidin MagneSphere® Paramagnetic Particles

Z548F 3 x 3ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Specifications

You are viewing: 3 isolations Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Nuclease-Free Water

P119C 3 x 25ml View Product

SSC, 20X

Z525A 3 x 1.4ml

Biotinylated Oligo(dT) Probe

Z526A 1 x 35μl View Product

mRNA User Tube

Z528A 3 x 1 each

MagneSphere® Technology Magnetic Separation Stand (two-position)

Z5333 1 x 12 × 75mm

Streptavidin MagneSphere® Paramagnetic Particles

Z548F 3 x 3ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Specifications

You are viewing: 15 isolations Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Nuclease-Free Water

P119C 2 x 25ml View Product

SSC, 20X

Z525A 2 x 1.4ml

Biotinylated Oligo(dT) Probe

Z526B 1 x 50μl View Product

MagneSphere® Technology Magnetic Separation Stand (two-position)

Z5332 1 x 1.5ml

Streptavidin MagneSphere® Paramagnetic Particles

Z548C 15 x 0.6ml

SDS

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Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Specifications

You are viewing: 15 isolations Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Nuclease-Free Water

P119C 2 x 25ml View Product

SSC, 20X

Z525A 2 x 1.4ml

Biotinylated Oligo(dT) Probe

Z526B 1 x 50μl View Product

Streptavidin MagneSphere® Paramagnetic Particles

Z548C 15 x 0.6ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Resources

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RNA Analysis Workflow

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