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Riboprobe® Combination Systems

Produce High-Specific-Activity RNA Probes or Defined RNA Transcripts

  • Choose from combination systems with T3/T7 or SP6/T7 RNA polymerases
  • SP6, T7 and T3 RNA Polymerases are extremely promoter-specific, allowing production of virtually homogeneous RNA using plasmid DNA as a template
  • Allows synthesis of RNA corresponding to the coding or noncoding strand of cloned DNA from a single plasmid construct

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Catalog number selected: P1450

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Riboprobe® Combination Systems
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$ 295.00
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The Riboprobe® Combination Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. The Riboprobe® Combination Systems include the RNA polymerases, all of the required reagents (excluding radioisotope) for performing transcription reactions in vitro and RQ1 RNase-Free DNase (Cat.# M6101) for removal of the template following transcription.

Applications of the Riboprobe® Systems include:

  • Generation of high-specific-activity RNA probes.
  • Generation of microgram quantities of RNA transcripts.
  • Generation of RNA processing substrates.
  • Generation of antisense RNA.

Protocols

Specifications

You are viewing: P1450 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

RQ1 RNase-Free DNase

M610C 1 × 110u View Product

Recombinant RNasin® Ribonuclease Inhibitor

N251A 1 × 2,500u40u/μl

rATP

P113A 2 × 50μl10mM View Product

rCTP

P114A 2 × 50μl10mM View Product

rGTP

P115A 2 × 50μl10mM View Product

rUTP

P116A 2 × 50μl10mM View Product

DTT

P117A 2 × 100μl100mM View Product

Transcription Optimized 5X Buffer

P118B 1 × 500μl View Product

Nuclease-Free Water

P119A 2 × 1,250μl View Product

T7 RNA Polymerase

P207D 1 × 500u

T3 RNA Polymerase

P208A 1 × 500u

pGEM® Express Positive Control Template

P256A 1 × 5μg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

You are viewing: P1460 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

RQ1 RNase-Free DNase

M610C 1 × 110u View Product

Recombinant RNasin® Ribonuclease Inhibitor

N251A 1 × 2,500u40u/μl

rATP

P113A 2 × 50μl10mM View Product

rCTP

P114A 2 × 50μl10mM View Product

rGTP

P115A 2 × 50μl10mM View Product

rUTP

P116A 2 × 50μl10mM View Product

DTT

P117A 2 × 100μl100mM View Product

Transcription Optimized 5X Buffer

P118B 1 × 500μl View Product

Nuclease-Free Water

P119A 2 × 1,250μl View Product

SP6 RNA Polymerase

P108E 1 × 500u

T7 RNA Polymerase

P207D 1 × 500u

pGEM® Express Positive Control Template

P256A 1 × 5μg

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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