TLR Reporter Bioassay

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Measure Potency and Stability of Molecules that Modulate TLR Signaling

  • 96- and 384-well plate formats
  • No primary cell culture required
  • Supplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format

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$ 1,440.00
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TLR Reporter Bioassay
Thaw-and-Use/1X each component
$ 1,440.00
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Simple Bioassay, Quality Results

The human immune system responds to conserved damage- and pathogen-associated molecular patterns (DAMPs and PAMPs, respectively) via pattern-recognition receptors (PRRs). One such family of PRRs is the Toll-like receptor (TLR) family. In humans, there are 10 known TLRs, which enable the immune system to sense and respond to damage and danger signals. Modulation of the TLR system, either by agonism or antagonism, has the potential to alter immune and inflammatory responses. Blockade of TLRs may inhibit inflammatory responses while TLR agonists may promote immune responses against pathogens or cancer.

Current methods for assessing the activity of TLR modulators typically rely on primary monocyte-derived macrophages and measurement of functional endpoints, such as cytokine production. These assays are laborious and highly variable due to their reliance on donor cells, complex assay protocols and unqualified assay reagents. As a result, these assays are difficult to establish in a quality-controlled setting.

The TLR Bioassay is a bioluminescent reporter cell-based assay used to measure the potency and stability of TLR agonists.

Assay Principle

The TLR Reporter Bioassay measures the potency and stability of agonists designed to activate TLR signaling.

Representation of the TLR Reporter Bioassay.

Representation of the TLR Reporter Bioassay. TLR Bioassay Cells are a monocytic, THP-1 cell line with a stably integrated NanoLuc® (NL) luciferase reporter driven by TLR pathway-dependent response elements. Treatment with a TLR agonist results in promoter-driven luminescence that can be detected and quantified using Bio-Glo-NL™ Reagent.

Assay Workflow: PAMP Detection Made Easy

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Bioassay Products Formats: Standardized Reagents to Improve Your Results

  • Thaw-and-Use Kit: Recommended for routine use with customer-defined agonist.
  • Cell Propagation Model: Cryopreserved cells for propagation and long-term use. Recommended for routine use with customer-defined agonist.
  • Cell Bank: After CPM purchase, cell bank of 50 vials manufactured under Promega quality systems.

Cell Culture vs. Thaw-and-Use Workflow

Standard bioassay workflow with cultured cells
Improved bioassay workflow with thaw-and-use cells

Benefits of Thaw-and-Use for You:

  • Standardized reagents: Provides significant time and labor savings.
  • Off-the-shelf option: Reduces assay variability.
  • Large manufactured lot sizes: Easily amenable to high-throughput workflows.

TLR Bioassay: Detect Pyrogens with Precision

What is a pyrogen detection assay?

A pyrogen detection assay is a test used to identify substances that can cause inflammatory responses when introduced into the human body. These substances, known as pyrogens, can originate from bacteria, fungi, or other sources and are recognized by Toll-like receptors (TLRs) and pose serious safety risks when found in pharmaceutical products. Pyrogen detection assays ensure product safety by verifying that therapeutics are free from contaminants before they reach clinical or commercial use.

What are traditional options for pyrogen testing?

  • Limulus Amebocyte Lysate (LAL):
    • Uses horseshoe crab blood that is highly sensitive to endotoxin.
    • Limited utility to non-endotoxins and presents sustainability concerns.
  • Primary Cell-Based Cytokine Assays:
    • Uses primary monocytes to measure cytokine in response to pyrogen exposure.
    • Highly variable due to donor biology and lengthy protocols.
  • Rabbit Pyrogen Test (RPT):
    • Uses live rabbits to detect an immune response post injection.
    • Relies on live animals, limited accuracy, and poor reproducibility.

How does the TLR Bioassay provide an improved pyrogen detection assay?

  • Physiologically Relevant: Uses an engineered THP-1 monocytic cell line without donor variability.
  • Comprehensive Detection: Identifies both endotoxin and non-endotoxin pyrogens that signals through TLR 1,2,4,5,6.
  • Ethical and Sustainable: Eliminates the need for animal-based testing methods.
  • Thaw-and-Use Format: Streamlined, cell-based assay with standardized reagents for rapid results.
pyrogen-research

The Data Have Spoken:

Promega cell-based assays have been the market leader for years. See how the TLR Bioassay continues that tradition in new applications.

Application Data: Targeting TLR Pathways

The TLR Bioassay luminescent readout can be used in a wide variety of important applications where modulating the TLR system, either by agonism or antagonism, is critical in understanding immune and inflammatory responses. Applications include TLR signaling for vaccine adjuvant development, antitumor activity and host-pathogen interactions.

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TLR4 agonism measured using the TLR Reporter Bioassay. The thaw-and-use TLR Bioassay was used to compare the TLR4 agonism between LPS, MPLA and CRX-527 to show varying levels of signaling for adjuvant ranking.

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TLR signaling for enhancing antitumor effects. R848 (resiquimod) is a synthetic imidazoquinoline compound recognized by TLR7 and TLR8 to modify the immune response from immune cells, like macrophage.

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Bacterial pathogen-associated molecular patterns (PAMPs) trigger TLR signaling. Lipoteichoic acid (LTA) is an immunogenic PAMP found in the cell wall of many Gram-positive bacteria and recognized by TLR2. LTA from Staphylococcus aureus was used to generate the data.

Specifications

You are viewing: JA9011 Change Configuration

What's in the box?

Item Part # Size

TLR Bioassay Cells

GA132A 1 × 0.5ml

Fetal Bovine Serum

J121A 1 × 4ml

RPMI 1640 Medium

G708A 1 × 36ml

Bio-Glo-NL™ Luciferase Assay Buffer

J308A 1 × 10ml

Bio-Glo-NL™ Luciferase Assay Substrate

J309A 1 × 200μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use; (2) discovery, development and monitoring of biologic drugs and vaccines; (3) quality assurance testing of biologic drugs and vaccines; and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use" means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo-NL™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega's. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Specifications

You are viewing: JA9015 Change Configuration

What's in the box?

Item Part # Size

TLR Bioassay Cells

GA132A 5 × 0.5ml

Fetal Bovine Serum

J121A 5 × 4ml

RPMI 1640 Medium

G708A 5 × 36ml

Bio-Glo-NL™ Luciferase Assay Buffer

J308A 5 × 10ml

Bio-Glo-NL™ Luciferase Assay Substrate

J309A 5 × 200μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use; (2) discovery, development and monitoring of biologic drugs and vaccines; (3) quality assurance testing of biologic drugs and vaccines; and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use" means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo-NL™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega's. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Specifications

You are viewing: GA1322 Change Configuration

What's in the box?

Item Part # Size

TLR Bioassay Cells (CPM)

GA130A 2 × 1ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

XX

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use; (2) discovery, development and monitoring of biologic drugs and vaccines; (3) quality assurance testing of biologic drugs and vaccines; and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use" means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells except that recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo-NL™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega's. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Specifications

You are viewing: GA6010 Change Configuration

What's in the box?

Item Part # Size

TLR Bioassay Cells (CPM)

GA130A 50 × 1ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

XX

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use; (2) discovery, development and monitoring of biologic drugs and vaccines; (3) quality assurance testing of biologic drugs and vaccines; and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use" means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells except that recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo-NL™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega's. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

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