IL-6 is a member of the IL-6 cytokine family, which includes IL-11, leukemia inhibitory factor (LIF), oncostatin M, ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1) and cardiotrophin-like cytokine (CLC). All of these cytokines signal through gp130 and the STAT3 pathway. IL-6 is transiently secreted following tissue damage or stressors, including UV irradiation, reactive oxygen species, and microbial and viral agents. It is also one of the cytokines released during bacterial sepsis. Furthermore, IL-6, along with transforming growth factor β (TGFβ), promotes differentiation of CD4+ T cells into Th17 cells and inhibits differentiation of regulatory T cells, thus playing a critical role in autoimmunity.
The IL-6 Bioassay is a bioluminescent cell-based assay designed to measure IL-6 stimulation or inhibition. The IL-6 Bioassay Cells have been engineered to express luc2P in response to IL-6 signaling. When IL-6 binds to IL-6 Bioassay Cells, the receptor transduces intracellular signals, resulting in luminescence. The bioluminescent signal is detected and quantified using Bio-Glo™ Reagent.