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FuGENE® HD Transfection Reagent

E2311

Better Transfection for Challenging Cell Lines

  • Difficult-to-transfect cell lines
  • Transfection of primary cells
  • Protein and virus production

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Catalog number selected: E2311

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FuGENE® HD Transfection Reagent
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$ 472.00
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32620075-FuGeneHD_OverviewHero

Transfect Cancer Model Cell Lines, Insect Cells and Stem Cells

Use the Best Cell Line for Your Study

FuGENE® HD Transfection Reagent offers unrivaled transfection performance with minimal impact on cell physiology (Jacobson et al. [2009] Biotechniques 47, 617–24). It is ideal for use in a variety of challenging applications, including transfection of cancer model cell lines, insect cells, stem cells, and for virus and protein production.

FuGENE® HD gives more biologically relevant results by allowing you to use the best cell line for your study, and doesn’t require harsh treatment of the cells by lowering serum concentration or washing of toxic lipid complexes. The in vivo environment even can be mimicked with transfection in 100% serum.

Read the Paper
32620075-FuGeneHD_Paper_977x1376

Unrivaled Transfection Performance

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Transfection of Stem Cells

“FuGENE® HD resulted in the highest stable transfection rate, estimated by antibiotic selection, with plasmids containing genes under the control of the EF1alpha or PGK promoters”.

Liu J., et al. (2009) Stable transgene expression in human embryonic stem cells after simple chemical transfection. Mol. Reprod. Dev. (2009) 76, 580-6.

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 Transfection of Cancer Cell Models

FuGENE® HD Transfection Reagent lets you work in your cancer model. Use the FuGENE® HD protocol database to see if transfection conditions for your cell line are available. The database includes over 25 cancer models and provides transfection protocols specific for each cell line.

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 Cellular Imaging

High expression levels and the low toxicity of FuGENE® HD can bring better performance for imaging experiments. Read more about the low toxicity of FuGENE® HD in:

Antczuk et al. (2014) A High Content Assay to Assess Cellular Fitness. Comb Chem High Throughput Screen. 17(1), 12–24.

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Protein Production

FuGENE® HD has no animal components and is compatible with defined media. This means you can conduct experiments on eukaryotic or mammalian cells with confidence.

High Expression with Minimal Effect on Cell Health

This figure compares transfection reagent performance in HEK293 cells. Cells were transfected with a luciferase reporter plasmid, and luciferase activity detected using the ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay. FuGENE® HD Transfection Reagent gave the highest expression levels and lowest toxicity.
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Ready-to-Use Transfection Protocols

Search our FuGENE® HD Protocol Database if you’re new to the reagent or exploring the use of a new cell line. We may already have a protocol for you!

These protocols guide the transfection process for many cell lines, and will also calculate the volumes needed based on well format and the number of samples.

Search for Protocols
32620075-FuGeneHD_Tool_ProtocolDatabase

Protocols

Specifications

You are viewing: 1ml Change Configuration

What's in the box?

Item Part # Size

FuGENE® HD Transfection Reagent

E231A 1 x 1ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Patents and Disclaimers

FuGENE is a registered trademark of Fugent, L.L.C., USA.

FuGENE® HD is sold with a Limited Use Label License (LULL).
You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.

Specifications

You are viewing: 5 × 1ml Change Configuration

What's in the box?

Item Part # Size

FuGENE® HD Transfection Reagent

E231A 5 x 1ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Patents and Disclaimers

FuGENE is a registered trademark of Fugent, L.L.C., USA.

FuGENE® HD is sold with a Limited Use Label License (LULL).
You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.

Resources

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