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FluoroTect™ GreenLys in vitro Translation Labeling System

L5001_FluoroTect GreenLys in vitro Translation Labeling System_3
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Fluorescent Labeling and Detection of in Vitro Synthesized Proteins

  • Data in minutes versus overnight exposures associated with radioactivity-based systems
  • "In-gel" detection; No need to transfer, fix or dry gels
  • No radioactivity required

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Catalog number selected: L5001

$ 514.00
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FluoroTect™ GreenLys in vitro Translation Labeling System
40 reactions
$ 514.00
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This system enables fluorescent labeling and detection of in vitro synthesized proteins, based on a lysine-charged tRNA labeled with the fluorophore BODIPY®-FL at the ε position. Fluorescent lysine residues are incorporated into synthesized proteins during in vitro translation reactions, eliminating the need for radioactivity.

Detection of the labeled proteins is accomplished in 2–5 minutes directly "in-gel" by use of a laser-based fluorescent gel scanner. This eliminates any need for protein gel manipulation such as fixing/drying or any safety, regulatory and waste disposal issues associated with the use of radioactively labeled amino acids. The convenience of "in-gel" detection also avoids the time-consuming electroblotting and detection steps of conventional non-isotopic systems.

The modified charged tRNA can be used with a variety of Promega translation systems including: Rabbit Reticulocyte Lysate, TnT® Coupled Transcription/Translation System, Wheat Germ Extract and E. coli S30 Extract.

For more information, see the Protocols & Applications Guide.

Schematic illustrating the incorporation of FluoroTect™ Green<sub>Lys</sub>-labeled lysine into nascent protein.

Schematic illustrating the incorporation of FluoroTect™ GreenLys-labeled lysine into nascent protein.

Specifications

What's in the box?

Item Part # Size

FluoroTect™ GreenLys tRNA

L500A 1 × 40μl

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

FluoroTect™ GreenLys incorporates Fluorotag® technology, which is licensed under U.S. Pat. Nos. 6,306,628 and 7,252,932. Commercial use of this product requires a license from AmberGen, Inc. Fluorotag is a registered trademark of AmberGen, Inc.

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