TMB One Solution is a chromagen substrate, 3,3´,5,5´-tetramethylbenzidine (TMB) provided in a mildly acidic, nonhazardous buffer for horseradish peroxidase detection in an ELISA format. The substrate is provided as a ready-to-use working dilution in a slightly acidic, nonhazardous proprietary buffer without aprotic solvents. TMB One Solution is noncaustic to plastics used in automated systems. The substrate develops a blue reaction product when oxidized by peroxidase and a yellow reaction product in an endpoint multiwell assay after the addition of an acid solution provided by the end user. TMB One Solution is stable for 12 months at 4°C, providing extended shelf life; the assay end product is stable for at least one hour after stopping the assay.
Note: Not recommended for Western analysis or immunohistochemistry.