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TMB One Solution

Substrate for Horseradish Peroxidase Detection in ELISAs

  • Single solution provided ready-to-use; just add, incubate, stop and read
  • Low background provides greater assay sensitivity

Size

Catalog number selected: G7431

$ 125.00
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TMB One Solution
100ml
$ 125.00
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TMB One Solution is a chromagen substrate, 3,3´,5,5´-tetramethylbenzidine (TMB) provided in a mildly acidic, nonhazardous buffer for horseradish peroxidase detection in an ELISA format. The substrate is provided as a ready-to-use working dilution in a slightly acidic, nonhazardous proprietary buffer without aprotic solvents. TMB One Solution is noncaustic to plastics used in automated systems. The substrate develops a blue reaction product when oxidized by peroxidase and a yellow reaction product in an endpoint multiwell assay after the addition of an acid solution provided by the end user. TMB One Solution is stable for 12 months at 4°C, providing extended shelf life; the assay end product is stable for at least one hour after stopping the assay.

Application

  • ELISA.

Note: Not recommended for Western analysis or immunohistochemistry.

Specifications

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

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