Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

TMB One Solution

Simpler protein detection banner-1250x300

Substrate for Horseradish Peroxidase Detection in ELISAs

  • Single solution provided ready-to-use; just add, incubate, stop and read
  • Low background provides greater assay sensitivity


Catalog number selected: G7431

$ 129.00
Your price:
Please Enquire
This product is discontinued
Add to Helix
TMB One Solution
$ 129.00
Your price: Log in

TMB One Solution is a chromagen substrate, 3,3´,5,5´-tetramethylbenzidine (TMB) provided in a mildly acidic, nonhazardous buffer for horseradish peroxidase detection in an ELISA format. The substrate is provided as a ready-to-use working dilution in a slightly acidic, nonhazardous proprietary buffer without aprotic solvents. TMB One Solution is noncaustic to plastics used in automated systems. The substrate develops a blue reaction product when oxidized by peroxidase and a yellow reaction product in an endpoint multiwell assay after the addition of an acid solution provided by the end user. TMB One Solution is stable for 12 months at 4°C, providing extended shelf life; the assay end product is stable for at least one hour after stopping the assay.


  • ELISA.

Note: Not recommended for Western analysis or immunohistochemistry.


Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


Let's find the product that meets your needs.

Talk to a Scientist