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Kazusa Collection

 

Kazusa Genome Technology (KGT)

Kazusa Genome Technology (KGT) is an academic Japanese research institute dedicated to genome-scale studies and the function of individual genes. The Kazusa ORFeome Project was initiated to create a collection of human and mouse ORFs to enable the larger research community with a readily accessible, highly validated ORFeome. KGT is an active member of the global ORFeome Collaboration, and are experts in HUGE (Human Unidentified Gene-Encoded) large proteins (>4kb). Promega and KGT have established a partnership, allowing Promega customers access to Kazusa's coveted collection of experimentally validated clones.

Experimentally-Validated Collection

Kazusa understands the value of extensive validation, and provides this data to their customers for the HaloTag® ORF collection. Check out the representative data pieces below, which are available for nearly all HaloTag®-fusion ORFs before you order.

Figure 9657TA 9658TA_200px

Fluorescent Microscopy demonstrating in situ labeling and visualization of HaloTag® fusions with HaloTag® TMR Ligand

Expression Validation by SDS-PAGE analysis of expressed HaloTag®-fusions in HEK293 cell lysates

Figure 11878TA Figure 11879TA

Insert Size Confirmation through agarose gel electrophoresis

Sequence Validation of both the 5' and 3' end sequences by single-pass sequencing

 

Overview of the Collection

Features HaloTag® Collection
PID beginning with FHC
Flexi® Collection
PID beginning with FXC
Size of Collection >9000 >6300
Fusion Tag HaloTag® for protein purification, imaging and pull-downs Un-tagged
Validated Clones
Sequence Validated 100% clones
Insert Validated 99.9% clones 97.2%
Expression Validated 99.2% clones
Imaging Validated 78% clones
Format DNA DNA
Typical Delivery 4-6 weeks 4-6 weeks

Discounts are available for bulk orders of multiple ORF clones (purified DNA).

  • 10% discount by ordering 20-49 clones
  • 20% discount by ordering more than 50 clones

Inquire at proteomics@promega.com before placing an order. Alternatively consider purchasing of an arrayed gene panel of HaloTag ORF clones as a bacterial glycerol stock.

 

The HaloTag® human full-length ORF clones are also available as premade panels of functionally related genes and as the entire 9000+ HaloTag® ORF clone library. These clones are supplied as pre-transformed glycerol stocks of E. coli with the same high quality standards associated with the single ORF clones available through FindMyGene™. Each clone is supplied as a glycerol stock in either a standard 96-well plate or in the 96 well Matrix plate. The Matrix plate is composed of 96 individually-capped tubes, where each tube is 2D-barcoded and uniquely numbered to allow ORF clone handling either as individual tubes one-at-a-time, or at-once as an entire library/panel using an automated liquid handler (e.g., with proper attachments).

well_plate
 

All orders are accompanied by a detailed documentation that describes the location of each clone. All clones were end-sequenced to ensure greater than 99.9% accuracy for clone position and correspondence to tube ID. Gene categorization was based primarily on Gene Ontology (GO) annotations. Additional information for some of the ORF categories was obtained from various review articles and other publicly available databases.

 
Available Gene Library/Panel
(click to see the panel in detail)
Available Genes
The Entire HaloTag human ORF clone library 9,020
Transcription Factors 930
Kinase 800
Epigenetics-Related 600
Ubiquitin-Related 750
RNA Binding 480
Cytokines 330
Growth Factors 280
Proteases 270
Phosphatases 90
GPCRs 640
Transporters 430
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