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ImProm-II™ Reverse Transcription System

A3800

Reverse Transcribe RNA Templates Up to 8.9kb

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Catalog number selected: A3800

$ 466.00 Your price: Log In

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ImProm-II™ Reverse Transcription System
100 reactions
$ 466.00
Your price: Log In

Complete System for First-Strand cDNA Synthesis of Long or Rare Messages

The ImProm-II™ Reverse Transcription System produces efficient, robust synthesis of first-strand cDNA in preparation for PCR amplification. The components of the ImProm-II™ Reverse Transcription System can be used to reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. The optimized reaction buffer and powerful ImProm-II™ Reverse Transcriptase provided in the ImProm-II™ System together enable robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The cDNA synthesis conditions were formulated for standalone applications or for easy transition to gene-specific target amplification. An aliquot of the reverse transcription reaction (1–20μl) can be amplified directly using Taq DNA polymerase in coupled or uncoupled PCR.

Advantages
  • Amenable to Full-Length RT-PCR: Reverse transcribe long RNA templates up to 8.9kb.
  • Microarray-Compatible: May be used to incorporate regular, Cy®3-modified, Cy®5-modified and amino-allyl-modified nucleotides.
  • Easy to Use: System provides all reagents necessary for efficient reverse transcription.
  • Scalable and Flexible: 1–20μl of the initial reverse transcription reaction may be used in subsequent PCR, and the optimized buffer allows coupled RT-PCR.
  • RT Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl and 50mM DTT. A 25mM MgCl2 Solution also is included.
  • Versatile: Use with your thermostable DNA polymerase of choice.
  • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Protocols

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Upstream Control Primer

A109B 1 × 100μl15μM

Downstream Control Primer

A110B 1 × 100μl15μM

MgCl2

A351H 1 × 1.2ml25mM View Product

Oligo(dT)15 Primer

C110B 1 × 50μg500μg/ml View Product

dNTP Mix

C114B 1 × 320μl10mM

Random Primers

C118B 1 × 50μg View Product

1.2kb Kanamycin Positive Control RNA

C138A 1 × 5μg0.5mg/ml View Product

ImProm-II™ 5X Reaction Buffer

M289A 1 × 600μl

ImProm-II™ Reverse Transcriptase

M314A 1 × 100μl

Recombinant RNasin® Ribonuclease Inhibitor

N251A 1 × 2,500u40u/μl

Nuclease-Free Water

P119A 2 × 1,250μl View Product

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

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