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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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The ImProm-II™ Reverse Transcription System produces efficient, robust synthesis of first-strand cDNA in preparation for PCR amplification. The components of the ImProm-II™ Reverse Transcription System can be used to reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. The optimized reaction buffer and powerful ImProm-II™ Reverse Transcriptase provided in the ImProm-II™ System together enable robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The cDNA synthesis conditions were formulated for standalone applications or for easy transition to gene-specific target amplification. An aliquot of the reverse transcription reaction (1–20μl) can be amplified directly using Taq DNA polymerase in coupled or uncoupled PCR.
ImProm-II™ Reverse Transcription System Technical Manual
PDF (1 MB)
Upstream Control Primer
Downstream Control Primer
1.2kb Kanamycin Positive Control RNA
ImProm-II™ 5X Reaction Buffer
ImProm-II™ Reverse Transcriptase
Recombinant RNasin® Ribonuclease Inhibitor
PDF (0 B)
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See Protocol for detailed storage recommendations.
U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.
Autophagy modulates amino acid signaling network in myotubes: differential effects on mTORC1 pathway and the integrated stress response.
Dye-based RT-qPCR kit for reverse transcription and amplification in one step.
Dye-based RT-qPCR kit for separate reverse transcription and amplification reactions.
Robust native Taq DNA polymerase with room-temperature setup.
M5001, M5005, M5006, M5008
Transcribes cDNA from RNA in as little as 15 minutes.
Optimized, dependable reverse transcription. Available as kit, master mix or standalone enzyme.
A5003, A5004, A5000, A5001, A2790, A2791, A2800, A2801
Individual 100mM solutions of dATP, dTTP, dGTP and dCTP.
U1205, U1202, U1201, U1215, U1211, U1212, U1225, U1221, U1222, U1235, U1231, U1232
Simple spin or vacuum-based method to extract total RNA from tissues, cultured cells and white blood cells.
Z3101, Z3100, Z3105
The most-trusted product for broad-spectrum inhibition of common eukaryotic RNases.
N2111, N2115, N2511, N2515, N2611, N2615
Purifies RNA from cells using a low elution volume and the Maxwell® RSC Instrument.
Purifies RNA from 1–16 tissue samples using a low elution volume and the Maxwell® RSC Instrument.
Purifies RNA from 1–16 whole blood samples using a low elution volume and the Maxwell® RSC Instrument.
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