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ImProm-II™ Reverse Transcription System

Reverse Transcribe RNA Templates Up to 8.9kb

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Catalog number selected: A3800

$ 514.00
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ImProm-II™ Reverse Transcription System
100 reactions
$ 514.00
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Complete System for First-Strand cDNA Synthesis of Long or Rare Messages

The ImProm-II™ Reverse Transcription System produces efficient, robust synthesis of first-strand cDNA in preparation for PCR amplification. The components of the ImProm-II™ Reverse Transcription System can be used to reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. The optimized reaction buffer and powerful ImProm-II™ Reverse Transcriptase provided in the ImProm-II™ System together enable robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The cDNA synthesis conditions were formulated for standalone applications or for easy transition to gene-specific target amplification. An aliquot of the reverse transcription reaction (1–20μl) can be amplified directly using Taq DNA polymerase in coupled or uncoupled PCR.

Advantages
  • Amenable to Full-Length RT-PCR: Reverse transcribe long RNA templates up to 8.9kb.
  • Microarray-Compatible: May be used to incorporate regular, Cy®3-modified, Cy®5-modified and amino-allyl-modified nucleotides.
  • Easy to Use: System provides all reagents necessary for efficient reverse transcription.
  • Scalable and Flexible: 1–20μl of the initial reverse transcription reaction may be used in subsequent PCR, and the optimized buffer allows coupled RT-PCR.
  • RT Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl and 50mM DTT. A 25mM MgCl2 Solution also is included.
  • Versatile: Use with your thermostable DNA polymerase of choice.
  • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Specifications

What's in the box?

Item Part # Size Concentration Available Separately

Upstream Control Primer

A109B 1 × 100μl 15μM

Downstream Control Primer

A110B 1 × 100μl 15μM

MgCl2

A351H 1 × 1.2ml 25mM View Product

Oligo(dT)15 Primer

C110B 1 × 50μg 500μg/ml View Product

dNTP Mix

C114B 1 × 320μl 10mM View Product

Random Primers

C118B 1 × 50μg View Product

1.2kb Kanamycin Positive Control RNA

C138A 1 × 5μg 0.5mg/ml View Product

ImProm-II™ 5X Reaction Buffer

M289A 1 × 600μl

ImProm-II™ Reverse Transcriptase

M314A 1 × 100μl

Recombinant RNasin® Ribonuclease Inhibitor

N251A 1 × 2,500u 40u/μl View Product

Nuclease-Free Water

P119A 2 × 1,250μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

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