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Access RT-PCR System

A1250
PCR Satisfaction Guaranteed

Convenient RT-PCR in One Tube

  • All the reagents you need for single-tube reverse transcription and PCR
  • Separate components allow you to control every step of the reaction

Size

Catalog number selected: A1260

$ 144.00 Your price: Log In

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Access RT-PCR System
20 reactions
$ 144.00
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Separate Components to Assemble Your Single Tube RT-PCR

The Access RT-PCR System is designed for the reverse transcription (RT) and PCR amplification of a specific target RNA from total RNA or mRNA. This one-tube, two-enzyme system provides sensitive, quick and reproducible analysis of even rare RNAs. The system uses AMV Reverse Transcriptase (AMV RT) from Avian Myeloblastosis Virus for first-strand DNA synthesis and thermostable Tfl DNA polymerase from Thermus flavus for second-strand cDNA synthesis and DNA amplification. The Access RT-PCR System includes an optimized single-buffer system that permits extremely sensitive detection of RNA transcripts without a requirement for buffer additions between the reverse transcription and PCR amplification steps. This simplifies the procedure and reduces the potential for contaminating the samples. In addition, the improved performance of AMV Reverse Transcriptase at elevated temperatures in the AMV/Tfl 5X Reaction Buffer minimizes problems encountered with secondary structures in RNA.


  • Maximum Control: Separate tubes of each component allow you to control every step of the reaction. You can optimize Mg2+ and perform no-reverse transcriptase control reactions.
  • Less Template: Detect message from as little as 1pg of total RNA or mRNA.
  • No Buffer Additions Required: The AMV/Tfl 5X Reaction Buffer results in optimal enzyme activity without buffer additions between the reverse transcription and DNA amplification steps.
  • Performance-Tested System: Promega PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.
  • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

For more information, see the Protocols & Applications Guide.


  1. Miller, K. and Storts, D.R. (1995) Promega Notes 53, 2–5.
  2. Kaledin, A.S. et al. (1981) Biokhimiia 46, 1576–84.

Protocols

Specifications

You are viewing: A1260 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Upstream Control Primer

A109B 1 × 100μl15μM

Downstream Control Primer

A110B 1 × 100μl15μM

Positive Control RNA with Carrier

A111A 1 × 50μl62.5amol

AMV Reverse Transcriptase

A261B 1 × 100u

AMV/Tfl 5X Reaction Buffer

A808A 1 × 1ml

dNTP Mix

C114F 1 × 20μl10mM

MgSO4

M197A 1 × 1,250μl25mM

Tfl DNA Polymerase

M391A 1 × 100u

Nuclease-Free Water

P119E 1 × 13ml View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research, commercial or diagnostic applications without any license or royalty fees.

Specifications

You are viewing: A1250 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Upstream Control Primer

A109B 1 × 100μl15μM

Downstream Control Primer

A110B 1 × 100μl15μM

Positive Control RNA with Carrier

A111A 1 × 50μl62.5amol

AMV Reverse Transcriptase

A261A 1 × 500u

AMV/Tfl 5X Reaction Buffer

A808A 1 × 1ml

dNTP Mix

C114A 2 × 50μl10mM

MgSO4

M197A 1 × 1,250μl25mM

Tfl DNA Polymerase

M391C 1 × 500u

Nuclease-Free Water

P119E 1 × 13ml View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research, commercial or diagnostic applications without any license or royalty fees.

Specifications

You are viewing: A1280 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Upstream Control Primer

A109B 1 × 100μl15μM

Downstream Control Primer

A110B 1 × 100μl15μM

Positive Control RNA with Carrier

A111A 1 × 50μl62.5amol

Access RT-PCR Core Reagents (100 reactions; A1250 minus Controls)

A1281 4 × 1 each

AMV Reverse Transcriptase

A261A 1 × 500u

AMV/Tfl 5X Reaction Buffer

A808A 1 × 1ml

dNTP Mix

C114A 2 × 50μl10mM

MgSO4

M197A 1 × 1,250μl25mM

Tfl DNA Polymerase

M391C 1 × 500u

Nuclease-Free Water

P119E 1 × 13ml View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research, commercial or diagnostic applications without any license or royalty fees.

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