Maxwell® 16 Cell LEV Total RNA Purification Kit

AS1225_Maxwell-16-Cell-LEV-Total-RNA-Purification-Kit
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Maxwell® 16 Cell LEV Total RNA Purification Kit

High Yield, High-Concentration Total RNA from Cells

The Maxwell® 16 Cell LEV Total RNA Purification Kit is used with the Maxwell® 16 LEV Instrument. This RNA purification procedure provides an easy method for efficient, automated purification of highly concentrated total RNA from cell samples. Total RNA can be isolated from cells grown either in suspension or attached to plates. Cells are collected and processed in up to 400μl volumes. Purified RNA for up to 16 samples is obtained in less than 45 minutes of hands-free instrument operation. No post-purification treatment with nuclease, cleanup or concentration is required to achieve superior performance in downstream applications. The low elution volume (30–100μl) is used to generate purified RNA in a more concentrated format for use in downstream applications such as qRT-PCR, RT-PCR and cDNA synthesis.

  • High yields and high-concentration total RNA result in better performance in gene expression analysis applications.
High concentration and yield using the Maxwell 16 Cell LEV Total RNA Purification Kit.

High concentration and yield using the Maxwell® 16 Cell LEV Total RNA Purification Kit. Total RNA was purified from 5 × 105, 7.5 × 105 or 1 × 106 HEK 293, NIH/3T3 or Jurkat cells and eluted in 50µl of Nuclease-Free Water. The average yield (µg) is shown as a dotted line, and the average concentration (ng/µl) is shown as a solid line. Maxwell® 16 Cell LEV Total RNA Purification Kit processing capacity depends on cell type, cell physiology and amount of cells used.

Specifications

What's in the box?

Item Part # Size Concentration Available Separately

LEV Plungers

AS610A 1 × 50/pk View Product

Elution Tubes (0.5ml)

AS620A 1 × 50/pk View Product

Maxwell® 16 LEV RNA Cartridge

AS640A 48 × 1 each

RNasin® Plus RNase Inhibitor

N261B 1 × 10,000u 40u/μl

Nuclease-Free Water

P119C 1 × 25ml View Product

RNA Lysis Buffer (RLA)

Z3051 1 × 50ml View Product

RNA Dilution Buffer (RDB)

Z372A 1 × 44ml

CytoSol Buffer

Z411A 1 × 10ml

Beta-Mercaptoethanol

Z559A 1 × 900μl

Certificate of Analysis

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

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