Maxwell® HT ccfDNA Kit

Ready-to-Go ccfDNA Extraction Kit, Expert Support Available

The Maxwell® HT ccfDNA Kit is compatible with automation on all major platforms, including Hamilton Star/Nimbus, Tecan Freedom Evo/Fluent, Biomek i5/i7, Eppendorf epMotion, and particle movers such as KingFisher.

When you purchase a Promega extraction solution, you gain free access to our elite Field Support Scientists, method development services, and technical support. Contact us if you need help setting up the Maxwell® HT ccfDNA Kit on your robot.

With decades of experience in building personalized automation solutions, we can help you customize the scale, application, and chemistry, regardless of platform, exactly to your needs—the only limit is your imagination.

Automated Purification of High-Quality, Amplifiable DNA

The Maxwell® HT ccfDNA Kit can be used for high-throughput, automated purification of circulating cell-free DNA (ccfDNA) from up to 96 samples of plasma with input volumes of 1.0–8.0ml.

Download Protocol

13589ma-w — **Quantitation of isolated DNA by qPCR.** DNA isolated from various input volumes using the Maxwell® HT ccfDNA Kit and the Qiagen QIAamp® ccfDNA/RNA kit is quantitated using qPCR. Yields using the Promega system are consistently higher when similar input volumes are used. Only the Promega system is capable of starting from as much as 8.0ml of input volume.

High-Throughput Automated ccfDNA Extraction from Plasma, Serum, Urine and CSF

The posters and application notes listed below contain protocols and example data using the Maxwell® HT ccfDNA Kit for automated purification of circulating, cell-free DNA from various body fluids on different robotic systems.

Circulating Cell-Free DNA Resources

ccfDNA Webinar Series

Register for one of our on-demand webinars to learn more about trends in ccfDNA research. From "What is ccfDNA?" to advanced applications, this series covers all the basics and more, with an emphasis on optimizing extraction and purification of circulating DNA.

Part 1: ccfDNA 101: Emerging Trends in Oncology Research

Part 2: ccfDNA Workflows: Honing in on the Target

Part 3: ccfDNA In the Lab: Optimizing Purification for Sequencing

Protocol

Maxwell® HT ccfDNA Kit Protocol

What's in the box?

Item	Part #	Size	Available Separately
Maxwell® Resin E	MC148B	1 × 20ml
Binding Buffer (BBC)	MC1362	1 × 450ml	View Product
Wash 1 (CWF)	A3091	1 × 125ml	View Product
Wash 2 (CWG)	A3101	1 × 150ml	View Product
PCR Elution Buffer (EBE)	MC151B	1 × 24ml	View Product
NGS Elution Buffer (EBF)	MC152B	1 × 24ml	View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. No. 6,855,499 and other patents.

Resources

Other Resources

Lab Automation Resource Center

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Maxwell® HT ccfDNA Kit

Automated High-Throughput Extraction of Cell-Free DNA from Plasma, Serum, Urine and CSF

Ready-to-Go ccfDNA Extraction Kit, Expert Support Available

Automated Purification of High-Quality, Amplifiable DNA

High-Throughput Automated ccfDNA Extraction from Plasma, Serum, Urine and CSF