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Microsatellite Instability (MSI) Analysis

A0001

Fluorescent Multiplex PCR-Based Detection Method

  • Amplifies five mononucleotide repeat markers for MSI-H determination
  • Co-amplification of highly polymorphic pentanucleotide repeats
  • For research on genomic instability and mismatch repair system in cancer studies

Size

Catalog number selected: MD1641

$ 1,979.00 Your price: Log In

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Microsatellite Instability (MSI) Analysis
100 reactions
$ 1,979.00
Your price: Log In

PCR-Based MSI Detection

The MSI Analysis System, Version 1.2, is a fluorescent multiplex PCR-based method detect microsatellite instability (MSI), a form of genomic instability. This instability is due to insertion or deletion of repeating units during DNA replication and failure of the mismatch repair system (MMR) to correct these errors. MSI analysis typically involves comparing allelic profiles of microsatellite markers generated by amplification from matching pairs of test samples, which may be MMR-deficient, and normal tissue samples. New alleles in the abnormal sample not found in the corresponding normal sample indicate the presence of MSI. MSI analysis can be used as a screening method to identify samples for further characterization. 

The MSI Analysis System, Version 1.2, includes fluorescently labeled primers (marker panel) for co-amplification of seven markers for analysis of the MSI-high (MSI-H) phenotype, including five nearly monomorphic mononucleotide repeat markers (BAT-25, BAT-26, MON0-27, NR-21 and NR-24) and two highly polymorphic pentanucleotide repeat markers (Penta C and Penta D). Amplified fragments are detected using an ABI PRISM® 310, 3100, 3100-Avant, 3130 or 3130xl Genetic Analyzer after spectral calibration.

4586TA-W
Analysis of MSI phenotype.
MSI panels and bins for GeneMapper® software can be downloaded for consistent data analysis. Panels and bins text files simplify and standardize data analysis by providing automated assignment of genotypes using GeneMapper® 4.0 software.

Protocols

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

K562 DNA High Molecular Weight

DD208A 1 x 3μg10ng/μl

Internal Lane Standard 600

DG107A 1 x 150μl View Product

Gold ST★R 10X Buffer

DM241C 1 x 300μl

MSI 10X Primer Pair Mix

MD174A 1 x 100μl

Nuclease-Free Water

P119A 1 x 1,250μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,238,863 and other patents and patents pending.

U.S. Pat. Nos. 7,749,706 and 7,902,343.

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