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Mass Spec-Compatible Yeast and Human Protein Extracts

Protein Extracts for Easier Mass Spec Instrument Monitoring

  • Choose human or yeast, digested or intact extract
  • Monitor LC and MS instrument performance
  • Use for method development and optimization

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Catalog number selected: V7461

$ 417.00
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Mass Spec-Compatible Yeast and Human Protein Extracts
Yeast/Digest; 100µg
$ 417.00
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mass spectormetry protein standards 32609644-L1

Yeast/Human Protein Extracts for Monitoring LC/MS

The mass spectrometry-compatible yeast and human protein extracts are designed for monitoring mass spec instrument performance. The extracts are predigested and cleaned up by solid-phase extraction for immediate use in liquid chromatography/mass spectrometry (LC-MS) analysis. 

Each extract is available in a pre-digested, tryptic peptide form, used primarily for instrument performance monitoring. The yeast and human extracts are also offered in an intact undigested form to provide a test material for optimizing mass spec protein sample preparation.

Extract reproducibility is assured by stringent control over the manufacturing process. Each protein extract is monitored by LC-MS to confirm composition and protein integrity.

protein standards for mass spec 32609644-L1

LC/MS Performance: Consistent Mass Spec Profiles

Promega extracts provide complex protein reference material that offers consistent peptide retention time, signal intensity and other critical performance parameters.
performance of mass spec standards 12271MA-w
Consistent mass spec profile of complex protein extracts. Each run used 1µg of human predigested protein extract injected into the instrument (Waters NanoAquity HPLC system interfaced to a Thermo Fisher Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer). Peptides were resolved with 2-hour gradient. Weekly monitoring with the human extract ensured consistent analytical performance of the instrument.
mass spec standard K562 digest isobaric tag 12272MA-w

A Model System for Method Development

These protein extracts provide ideal model systems for development and optimization of various sample prep methods, including isobaric tagging and enrichment.

In the example of isobaric tagging shown here, three aliquots of the human K562 predigested extract were labeled with three different TMT isobaric mass tag reagents (TMT126, TMT127 and TMT128) and mixed at a 10:5:1 ratio. Relative quantitative ratio between the mass tags was determined with an LTQ Orbitrap Velos Pro™ mass spectrometer. The data show good correlation between the expected and observed protein quantitation levels.

Enhanced Quality Control Ensures Reliable, Reproducible Performance

Lot-to-lot consistency of extracts is monitored by various protein and peptide qualitative and quantitative methods, including LC-MS and amino acid analysis. Our manufacturing process assures compatibility with LC-MS by monitoring nonspecific protein fragmentation, non-biological post-translational modifications and, for digested extracts, minimal undigested peptides.
Quality MeasurementMS-Compatible Human Protein Extract Digest (Cat.# V6951) HeLa Protein Digest Standard (Other Supplier)
Non-Biological Post-Translational Modifications

Deamidation Spectra: <12%

Oxidation Spectra: <5%

Carbamylation Spectra: <5%

Not tested; no specification

Oxidation Spectra: <10%

Carbamylation Spectra: <10%

Missed Cleavages
<10%
<10%

Peptide Quality

Quantification of amino acids in sample

Protein fragmentation: 1% or less

Matching spectra: >65%


A280

Not tested; no specification

Not tested; no specification

 

Lot-to-Lot Consistency

>12,462 unique peptides

Total proteins: >1805

Protein identification: >85% of 1,194 human core peptides

Quantitation reproducibility monitored by relative abundance of 10 reference proteins

LC-MS chromatogram conforms to reference

-

Ratio of peptide area to reference = 0.75–1.125

Not tested

Specifications

You are viewing: V7461 Change Configuration

What's in the box?

Item Part # Size

MS Compatible Yeast Protein Extract, Digest

V746A 1 × 100μg

SDS

Choose language:

Download SDSPDF (40 KB) – English (United States)

Certificate of Analysis

See all certificates for V7461

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Specifications

You are viewing: V7341 Change Configuration

What's in the box?

Item Part # SizeConcentration

MS Compatible Yeast Protein Extract, Intact

V734A 1 × 1mg10mg/ml

SDS

Choose language:

Download SDSPDF (41 KB) – English (United States)

Certificate of Analysis

See all certificates for V7341

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

Specifications

You are viewing: V6951 Change Configuration

What's in the box?

Item Part # Size

MS Compatible Human Protein Extract, Digest

V695A 1 × 100μg

SDS

Choose language:

Download SDSPDF (40 KB) – English (United States)

Certificate of Analysis

See all certificates for V6951

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Specifications

You are viewing: V6941 Change Configuration

What's in the box?

Item Part # SizeConcentration

MS Compatible Human Protein Extract, Intact

V694A 1 × 1mg10mg/ml

SDS

Choose language:

Download SDSPDF (41 KB) – English (United States)

Certificate of Analysis

See all certificates for V6941

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

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