Maxwell® HT XtractAll FFPE DNA/RNA Kit
One Plate. Four Workflows. Unlimited Insight.
- Total versatility from a single FFPE input. Choose DNA-only, RNA-only, total nucleic acid (TNA) or sequential DNA → RNA extraction—maximizing precious samples
- Hands-off, high-throughput speed. Set up in ≤15 minutes; automated run processes up to 96 samples in <3.5 hours
- Quality you can trust. Magnetic-bead chemistry yields intact fragments that power sensitive assays (ddPCR, qPCR, NGS) and recover long amplicons other kits miss
- Quantify with confidence. Fluorescence-based measurements align with amplification assays, reducing repeat testing
- Scalable chemistry. Uses the same extraction reagents as the Maxwell® RSC XtractAll FFPE DNA/RNA Kit (Cat.# AS1570)
Catalog Number:
Size
Fast, Flexible Nucleic-Acid Purification for High-Throughput Labs
Unlock more data from every FFPE section with a single, automation-ready kit that lets you choose DNA-only, RNA-only, total nucleic acids or sequential DNA-then-RNA workflows—no sample reruns required. Setup takes ~15 minutes, and deck-compatible protocols process up to 96 samples in under three hours, delivering high-yield, fragment-intact DNA and RNA ready for ddPCR, qPCR and NGS. Fluorescence quantitation aligns with downstream performance, so you can advance to analysis with confidence.
Pending automation configuration, up to 96 samples or more can be processed in one instrument run using the Maxwell® HT XtractAll FFPE DNA/RNA Kit.
Fast and Versatile FFPE DNA and RNA—Without Compromises!
The Maxwell® HT XtractAll FFPE DNA/RNA Kit significantly reduces sample extraction turnaround time with workflows of ~3.5 hours or less, depending on the selected protocol. With this kit, once FFPE samples are transferred to processing tubes, the entire process can be automated for fast, consistent and efficient extraction. This streamlined approach enables laboratories to reliably and quickly process samples, freeing up valuable time for other laboratory needs.
The Maxwell® HT XTractAll FFPE DNA/RNA Kit is compatible with automation on all major platforms.
When you purchase a Promega extraction solution, you gain free access to our elite Field Support Scientists, method development services, and technical support. Contact us if you need help setting up the Maxwell® HT XtractAll FFPE DNA/RNA Kit on your robot.
With decades of experience in building personalized automation solutions, we can help you customize the scale, application and chemistry, regardless of platform, exactly to your needs—the only limit is your imagination.
The Maxwell® HT XtractAll FFPE DNA/RNA Kit delivers unmatched flexibility with four workflow options. From one FFPE input sample, choose to extract: DNA only, RNA only, total nucleic acid in a single eluate, or sequential DNA and RNA extraction into separate eluates. This adaptability supports a diversity of applications without compromising on yield or quality of results, eliminating the need to perform multiple extractions and benefiting laboratories with limited FFPE sample material.
The Maxwell® XtractAll FFPE DNA/RNA chemistry provides consistent yield of DNA and RNA in each workflow. FFPE lung tumor (Panel A) and FFPE colon normal (Panel B) samples were extracted using the Maxwell® XtractAll FFPE DNA/RNA chemistry following the DNA (“DNA”), RNA (“RNA”), total nucleic acid (“TNA”) or sequential DNA and RNA (“Seq DNA” and “Seq RNA”) protocols. The yield of DNA and RNA was evaluated via amplification assays (102bp target for DNA, 100bp target for RNA). Data represent mean ±SD, N=6.
High-Quality DNA and RNA for Advanced Downstream Analysis Assays
The Maxwell® HT XtractAll FFPE DNA/RNA Kit delivers high-quality DNA and RNA from a variety of tissue types. The nucleic acids extracted using this kit demonstrate excellent integrity and high yields of DNA and RNA, especially for longer fragment lengths as compared to other FFPE extraction offerings.
Comparison of DNA and RNA yields from a variety of FFPE tissue samples. DNA and RNA were extracted from multiple types of FFPE tissue samples (breast, liver, colon, kidney, uterine, endometrial, lung, cervical) using the Maxwell® XtractAll FFPE DNA/RNA chemistry sequential DNA/RNA workflow and two other commercially available sequential FFPE DNA/RNA extraction kits. DNA yield was evaluated by amplification using the ProNex® DNA QC Assay (Cat.# NG1002) for short (75bp; Panel A) and long (300bp; Panel B) target amplicons. RNA was evaluated by amplification assay for short (100bp; Panel C) and long (171bp; Panel D) target amplicons. The Maxwell® XtractAll FFPE DNA/RNA chemistry shows equivalent or superior performance for both DNA and RNA yields when evaluated by amplification assay, especially for longer target amplicons. Data represent mean ±SD, N=6.
Comparison of FFPE DNA and RNA fragment size and quality from several extraction kits. DNA and RNA were extracted from breast FFPE tissue samples using the Maxwell® XtractAll FFPE DNA/RNA chemistry sequential DNA/RNA workflow and two other commercially available sequential FFPE DNA/RNA extraction kits. Panel A. DNA fragment size was evaluated using the Agilent TapeStation D5000 ScreenTape Assay. Panel B. RNA fragment size was evaluated using the Agilent TapeStation RNA ScreenTape Assay, and DV200 values are shown. N=6.
This level of quality and consistency makes the Maxwell® HT XtractAll FFPE DNA/RNA Kit an ideal choice for demanding applications and sensitive assays, such as digital PCR or NGS.
Performance of Maxwell® XtractAll FFPE DNA/RNA in digital PCR. RNA was extracted from lung tumor FFPE samples using the Maxwell® XtractAll FFPE DNA/RNA chemistry and tested in EML4-ALK mRNA Fusions Assay (Bio-Rad). A 2D amplitude plot is shown; successful detection of the positive gene fusions is identified.
Reliable Fluorescent Quantitation for Confident Downstream Assay Performance
Fluorescence dye-based quantitation assays, such as the QuantiFluor® ONE dsDNA System (Cat.# E4871) and QuantiFluor® RNA System (Cat.# E3310), are often used to quickly and conveniently assess the yield of extracted DNA and RNA. In contrast to other FFPE extraction offerings, DNA and RNA extracted with the Maxwell® HT XtractAll FFPE DNA/RNA Kit produces fluorescence dye quantitation results that more reliably predict success of the purified nucleic acids in amplification assays, such as qPCR and digital PCR. This improved concordance between fluorescence dye quantitation and amplification assays minimizes the risk of failed downstream assays, saving you both time and money.
Assessment of fluorescence- and amplification-based quantitation assay concordance for DNA and RNA extracted from FFPE tissue. DNA and RNA were extracted from eight different FFPE tissue samples using the Maxwell® XtractAll FFPE DNA/RNA chemistry and two other commercially available sequential FFPE DNA/RNA extraction kits. Panel A. DNA was quantified using fluorescence-based (QuantiFluor® ONE dsDNA System [Cat.# E4871]) and amplification-based (qPCR; 102bp amplicon) assays. Panel B. RNA was similarly quantified using fluorescence-based (QuantiFluor® RNA System [Cat.# E3310]) and amplification-based (RT-qPCR, 100bp amplicon) assays. The ratio of fluorescence- to amplification-based quantification was calculated and compared. In contrast to the other extraction kits, the Maxwell® XtractAll FFPE DNA/RNA chemistry demonstrated fluorescence-based quantitation readouts that more consistently predict DNA and RNA quantitation via amplification-based assays.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Lysis Buffer (LBA) |
MC1181 | 1 × 115ml | View Product |
DNase Wash (XAL) |
MC1621 | 1 × 165ml | View Product |
Binding Buffer (XAL) |
MC1611 | 1 × 260ml | View Product |
Buffer B (BWB) |
A3161 | 1 × 200ml | View Product |
Mineral Oil |
MC121C | 1 × 220ml | |
Proteinase K Solution |
MC1111 | 1 × 20ml | View Product |
MnCl2, 0.09M |
MC125B | 1 × 15ml | |
DNase I, Lyophilized |
Z358C | 4 × 1 vial | |
|
Nuclease-Free Water |
P1195 | 1 × 150ml | View Product |
MagneSil® RED |
A164C | 1 × 40ml | |
Buffer A (BWA) |
A3151 | 1 × 133ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
MagneSil® RED |
A164D | 1 × 11ml | |
Proteinase K (PK) Solution |
MC111A | 5 × 1ml | View Product |
Lysis Buffer (LBA) |
MC118B | 1 × 45ml | |
Mineral Oil |
MC121A | 3 × 25ml | |
MnCl2, 0.09M |
MC125A | 3 × 1ml | |
Binding Buffer (XAL) |
MC161A | 1 × 83ml | |
DNase Wash (XAL) |
MC162A | 1 × 57ml | |
DNase I, Lyophilized |
Z358C | 2 × 1 vial | |
|
Nuclease-Free Water |
P1195 | 1 × 150ml | View Product |
Buffer A (BWA) |
A3151 | 1 × 133ml | View Product |
Buffer B (BWB) |
A3161 | 1 × 200ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Articles
Software and Firmware
Other Resources
- Webinar: Automation Experts Answer Your FFPE, Liquid/Tissue Biopsy DNA and RNA Extraction Questions Live
- Webinar: Simplify Complex Nucleic Acid Extractions: Learn How To Build Walkaway Automated Methods for FFPE, ccfDNA, Blood, etc.
- Nucleic Acid Purification Application Notes Portal
- Lab Automation Resource Center
- Webinar: Maxwell® XtractAll FFPE DNA/RNA Kit: Versatile and Efficient Nucleic Acid Extraction from FFPE Tissues
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Frequently Used With
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"Add-and-read" quantitation of double-stranded DNA. Includes DNA-binding dye, lambda DNA and buffer.
E4871, E4870
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Fluorescent quantitation of small amounts of RNA in solution.
E3310
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Check the gDNA extracted from potentially degraded sources or ccfDNA samples for both quality and quantity.
NG1002, NG1003, NG1001
