Maxwell® DE System
Semi-Automated Medium- and High-Throughput Separation of Sperm and Non-Sperm Fractions of Sexual Assault Samples
- Automated washes reduce manual labor and errors in differential extraction
- Flexible protocol options to adapt the workflow to lab needs while processing up to 48 samples at once
- Recover sperm DNA with high efficiency
- No further purification of sperm and non-sperm lysates required for downstream use with PowerQuant® and PowerPlex® Systems
Catalog Number:
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Streamline Your Differential Extraction Workflow
Forensic labs require efficiency and precision, especially when handling complex samples from sexual assault cases. Manual methods for differential extraction are time-consuming, and their performance can vary depending on the individual analyst.
The Maxwell® DE System enables medium- and high-throughput separation of sperm DNA from casework samples with mixed cell types and eliminates the need for purification when quantification and STR-analysis are performed with PowerQuant® and PowerPlex® Systems. Automation provides consistent and reproducible results for forensic casework analysts who need to efficiently process sexual assault samples to reduce their lab’s SAEK backlog.
Promega forensic products are manufactured in alignment with the ISO 18385 standard. This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.
Reduce Hands-On Time
In today’s high-throughput lab environments, time is one of the most valuable—and limited—resources. The Maxwell® DE System significantly reduces hands-on time compared to manual nucleic acid extraction, freeing up staff to focus on critical tasks like data analysis, troubleshooting and reporting. Less pipetting means fewer errors, improved consistency and greater reproducibility—without sacrificing yield or quality. For labs juggling limited staff or scaling up sample volumes, that translates into operational efficiency and a lot less tedium at the bench.
Save Resources with Direct Compatibility
The DNA obtained using the Maxwell® DE System can be subsequently quantified using the PowerQuant® System and amplified using the PowerPlex® STR multiplex of your choice.
- Non-sperm fraction: Purification maximizes compatibility with downstream workflows, with an option to shorten the process.
- Sperm fraction: Ready for direct quantification and amplification; purify if needed based on sample quality.
The Maxwell® DE System includes all components necessary for differential extraction.
Profiles from Fractions Obtained with the Maxwell® DE System
Non-Sperm Fraction
Sperm Fraction
Profiles obtained from the non-sperm (left) and sperm (right) fractions of the same sample collected 24 hours post-coitus. Fractions were obtained using the Maxwell® DE System and amplified using the PowerPlex® 35GY System. The concentration of autosomal DNA detected in the non-sperm fraction with the PowerQuant® System was 55.2ng/µl. The [Auto]/[Deg] ratio for the non-sperm fraction was 2.8. The concentration of autosomal DNA detected in the sperm fraction with the PowerQuant® System was 0.85ng/µl. The [Auto]/[Y] ratio for the sperm fraction was 1.0, and the [Auto]/[Deg] ratio was 1.6.
Maxwell® Instruments Make Forensic Labs More Efficient
Automated DNA extraction saves laboratories time and labor costs and frees staff to work on casework analysis. Capable of processing up to 16 or 48 samples per run, depending on which instrument is being used, Maxwell® Instruments and Kits meet the varying throughput demands of forensic laboratories. With less manual pipetting, you not only cut down on human error but also boost consistency and reproducibility. Maxwell® Instruments don't just save time—they help labs do more with the resources they already have.
Explore the Maxwell® FSC Instrument for forensic labs.
Explore the Maxwell® RSC 48 Instrument for higher sample throughput.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
1-Thioglycerol |
A208B-C | 1 × 900μl | |
|
Proteinase K (PK) Solution |
MC500C | 4 × 1ml | View Product |
Maxwell® DE Resuspension Buffer |
A837A | 1 × 60ml | |
Nuclease-Free Water |
P119H | 1 × 25ml | View Product |
Maxwell® DE Lysis Buffer |
A836A | 1 × 30ml | |
Maxwell® DE Elution Buffer |
A838A | 1 × 20ml | |
Maxwell® DE Cartridges |
AS190A | 48 × 1 each | |
Maxwell® RSC Plunger Pack |
AS711A | 1 × 48/pack | |
DNA IQ™ Spin Baskets |
V122A | 1 × 50/pack | View Product |
Click-Fit Microtube, 1.5ml |
V474A | 1 × 100/pack | View Product |
Elution Tubes, 0.5ml |
AS720A | 1 × 50/pack | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
Resources
Software and Firmware
Related Products
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Frequently Used With
Maxwell® FSC Instrument
Benchtop instrument for automated DNA extraction from casework samples.
AS4600
Maxwell® RSC 48 Instrument
Benchtop instrument for automated nucleic acid purification of 1–48 samples. For Research Use Only.
AS8500
Casework Direct System
Rapidly process sexual assault evidence and low abundance DNA samples.
DC4560, DC4561
PowerQuant® System
5-color, 4-target probe-based qPCR assay.
PQ5002, PQ5008, DS1221
PowerPlex® Fusion 6C System
6-color co-amplification and detection of 27 loci.
DC2705, DC2720, DC2780
PowerPlex® 35GY System
First 8-dye STR multiplex; allows co-amplification and fluorescent detection of the 20 CODIS core loci plus Amelogenin and DYS391 for sex determination.
DC3520, DG5010, DM1551, DG8571
PowerPlex® Y23 System
5-dye STR multiplex for amplification of 23 Y-STR loci.
DC2305, DC2320, DM6571
