In a 1.5ml microcentrifuge tube, 400µl of preprocessing buffer is added to bone powder and incubated at 56ºC for 2.5 hours on a shaker set at 1000rpm. The tube is then centrifuged for 5 minutes at 13,000 × g, and the supernatant is transferred to a new 1.5ml microcentrifuge tube containing Lysis Buffer with a reducing agent. At this point, the preprocessed sample is ready for purification using the DNA IQ™ manual or DNA IQ™ Maxwell® method. The 1.5ml microcentrifuge tube with the undigested bone powder pellets can be discarded. Following the purification protocol, DNA can be eluted in 50µl of elution buffer. The resultant DNA can be quantified using the PowerQuant® System and amplified using the PowerPlex® Systems to generate an STR profile.
Integration of the Bone DNA Extraction Kit into a laboratory's workflow offers a rapid, cost-effective means to extract DNA with minimal hands-on time from precious bone samples.