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Bone DNA Extraction Kit, Custom

Rapidly Process Bone and Teeth Samples

  • Quick preprocessing of bone powder using Demineralization Buffer, Custom
  • Compatible with manual and Maxwell® DNA IQ™ chemistry
  • Yields DNA free of inhibitors and compatible with downstream amplification reactions

Introducing the fastest way to order everything you need.

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Catalog number selected: AX6780

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Bone DNA Extraction Kit, Custom
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Rapidly Process Challenging Skeletal Remains

In certain cases involving missing persons, mass disasters and anthropological excavations, bone and teeth are often the only sample types available for identification. Further, these samples are often subjected to environmental damage, and co-extraction of environmental inhibitors during purification limits their usability in STR typing experiments. Purification of DNA free of inhibitors is a crucial first step in determining the success of obtaining a usable profile from these samples.

A short preprocessing protocol followed by purification of DNA using DNA IQ™ chemistry yields inhibitor-free DNA that is compatible with PowerQuant® or Plexor® HY Systems for human and male DNA quantification and PowerPlex® STR Systems for generation of a STR profile. 

ForensicGrade

Promega forensic products are manufactured in alignment with the ISO 18385 standard.This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.

How It Works

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400µl of preprocessing buffer is added to bone powder in a 1.5ml microcentrifuge tube, incubated at 56ºC for 2.5 hours on a shaker set at 1000rpm. The tube is then centrifuged for 5 minutes at 13,000 × g and the supernatant is transferred to a new 1.5ml microcentrifuge tube containing Lysis buffer with a reducing agent. At this point, the preprocessed sample is ready for purification using the DNA IQ™ manual or DNA IQ Maxwell® method. The 1.5ml microcentrifuge tube with the undigested bone powder pellets can be discarded. Following the purification protocol, DNA can be eluted in 50µl of elution buffer and the resultant DNA can be quantified using the PowerQuant® System and amplified using the PowerPlex® Systems for generation of STR profile.

Integration of the Bone DNA Extraction Kit, Custom into a laboratory's workflow offers a rapid, cost effective means to extract DNA with minimal hands-on time from precious bone samples.

Specifications

What's in the box?

Item Part # SizeAvailable Separately

Lysis Buffer

A826B 1 × 150ml View Product

Demineralization Buffer, Custom

AX678A 1 × 50ml

Proteinase K (PK) Solution

MC500B 1 × 4ml View Product

1-Thioglycerol

A208B 3 × 900μl

Certificate of Analysis

See all certificates for AX6780

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

DD

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