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NAD(P)H-Glo™ Detection System

Monitor the Concentration of Reduced Forms of NADH and NADPH

  • Highly sensitive and rapid bioluminescent assay
  • Easily adapted for high-throughput format

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Catalog number selected: G9061

$ 361.00
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NAD(P)H-Glo™ Detection System
10ml
$ 361.00
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NADP_NADH_Background-Image

The NAD(P)H-Glo™ Detection System is a bioluminescent, homogeneous, single-reagent-addition assay that quantitatively monitors the concentration of the reduced forms of NADH and NADPH levels in enzymatic reactions. The oxidized forms, NAD+ and NADP+, are not detected and do not interfere with quantitation.

The assay is rapid, requiring only a 40- to 60-minute incubation, has a broad linear range (25nM-50µM) and high signal-to background ratio (~400). Low nanomolar sensitivity, 25nM, allows direct in-well detection. The assay is well suited to measuring NAD(P)H production or consumption in high-throughput formats.

Assay Chemistry

In the presence of NAD(P)H, a reductase reduces the proluciferin reductase substrate to form luciferin. Luciferin is quantified using Ultra-Glo™ Recombinant Luciferase, which is a component of the Luciferin Detection Reagent, and the light signal produced is proportional to the amount of NAD(P)H in the sample.

NADP_NADH_Foreground-Image

Sensitive and Selective

The assay is selective for the reduced forms of NADH and NADPH levels in enzymatic reactions.

Compatible with High-Throughput Systems

The simple add-and-read protocols are compatible with automated and high-throughput workflow, and the flexible chemistries adapt to 96-, 384- and 1,536-well plate formats and high-throughput systems. The yield Z′ values >0.7

Assays were performed using 8µl of the indicated concentration of NADH with 8µl of the appropriate Luciferin Detection Reagent in 384-well plates. S/B = signal-to-background ratio.

The NAD(P)H-Glo™ Assay detects only NADH and NADPH.

Easy to Use "Add-Mix-Measure" Protocol

Specifications

You are viewing: G9061 Change Configuration

What's in the box?

Item Part # SizeConcentration

Reductase

G884A 1 × 55μl6mg/ml

Reductase Substrate

G885A 1 × 55μl

Luciferin Detection Reagent

V859A 1 × 1 each

Reconstitution Buffer

V865A 1 × 10ml

SDS

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Download SDSPDF (131 KB) – English (United States)

Certificate of Analysis

See all certificates for G9061

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Patent Pending.

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Specifications

You are viewing: G9062 Change Configuration

What's in the box?

Item Part # SizeConcentration

Reductase

G884B 1 × 275μl6mg/ml

Reductase Substrate

G885B 1 × 275μl

Luciferin Detection Reagent

V859B 1 × 1 each

Reconstitution Buffer

V865B 1 × 50ml

SDS

Choose language:

Download SDSPDF (192 KB) – English (United States)

Certificate of Analysis

See all certificates for G9062

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Patent Pending.

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

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