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  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
  • Genome qualified to ensure optimal performance in genomic analysis applications

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Catalog number selected: R7103

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Cut Site:



  1. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
  2. Sensitive to CpG methylation.
  3. SgfI is inactivated by freezing at –70°C.
  4. Ends generated by SgfI can be directly ligated to ends generated by PvuI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.

Incubation Conditions: Buffer C. 37°C.

Source: Streptomyces fulvissimis.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1% Thesit®, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

25–50% 25–50% 100% <10% <10%

Frequency of Cutting:

λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
0 1 0 0 0 0


What's in the box?

Item Part # SizeConcentrationAvailable Separately

Buffer C 10X Buffer

R003A 1 × 1ml

Bovine Serum Albumin, Acetylated

R396D 1 × 150μl10mg/ml View Product


R710B 1 × 250u


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Download SDSPDF (91 KB) – English (United States)

Certificate of Analysis

See all certificates for R7103

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


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