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KpnI

R6011

  • Capable of digesting DNA in 15 minutes or less
  • Active and capable of digestion directly in GoTaq® Green Master Mix
  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
  • Available at high concentration, containing 12,500 units of KpnI at a concentration of 40–80u/μl

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Size

Catalog number selected: R6341

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KpnI
8–12u/µl/2,500u
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Cut Site:

G   GTACC
CCATG   G

Usage Notes:

  1. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
  2. KpnI recognizes the same DNA sequence but cleaves at a different nucleotide than its neoschizomer, Acc65I.
  3. KpnI is not sensitive to overlapping dcm methylation, unlike its neoschizomer, Acc65I.

Incubation Conditions: Buffer J. 37°C.

Source: Recombinant E. coli strain.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 200mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A B C D J MULTI-CORE™
100%* 25–50% 25–50% <10% 100% 75–100%

*Buffer A is not recommended due to potential star activity.

Frequency of Cutting:

λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
2 8 0 1 1 0

Protocols

Specifications

You are viewing: 2,500u Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Buffer J 10X Buffer

R009A 1 x 1ml

Bovine Serum Albumin, Acetylated

R396D 1 x 150μl10mg/ml View Product

KpnI

R634A 1 x 2,500u

MULTI-CORE™ 10X Buffer

R999A 1 x 0.25ml View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

You are viewing: 10,000u Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Buffer J 10X Buffer

R009A 1 x 1ml

Bovine Serum Albumin, Acetylated

R396E 1 x 750μl10mg/ml View Product

KpnI

R634G 1 x 10,000u

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

You are viewing: 12,500u Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Buffer J 10X Buffer

R009A 1 x 1ml

Bovine Serum Albumin, Acetylated

R396E 1 x 750μl10mg/ml View Product

KpnI (HC)

R434A 1 x 12,500u

MULTI-CORE™ 10X Buffer

R999A 1 x 0.25ml View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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