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pGEM®-5Zf(+) Vector

Standard Cloning Vector and Template for In vitro Transcription and Circular ssDNA Production

  • Blue/white screening to easily identify recombinant clones
  • Versatile vector is used for standard cloning, single-stranded DNA production and in vitro transcription
  • Multiple cloning site provides a convenient selection of restriction sites for cloning

Size

Catalog number selected: P2241

$ 131.00 Your price: Log In

pGEM®-5Zf(+) Vector
20μg
$ 131.00
Your price: Log In

The pGEM®-5Zf(+) Vector is derived from the pGEM®-3Zf(+) Vector and contains the origin of replication of the filamentous phage f1. This plasmid serves as a standard cloning vector, as a template for in vitro transcription and can be used for the production of circular ssDNA. This vector contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for ApaI, AatII, SphI, NcoI, SacII, EcoRV, SpeI, NotI, PstI, SalI, NdeI, SacI, BstXI and NsiI.

Reference

  1. Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
0284VC02_5A-W

pGEM®-5Zf(+) Vector  GenBank® Accession Number X65308 and vector sequence text file.

Specifications

What's in the box?

Item Part # SizeAvailable Separately

pGEM®-5Zf(+) Vector

P224A 1 × 20μg

Bacterial Strain JM109

P975A 1 × 500μl View Product

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for storage recommendations.

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