We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Use code TVECTOR20 at checkout to get 20% off pGEM®-T Vector Systems! Shop Now ›

pGEM®-5Zf(+) Vector


Standard Cloning Vector and Template for In vitro Transcription

  • Blue/white screening to easily identify recombinant clones
  • Multiple cloning site provides a convenient selection of restriction sites for cloning


Catalog number selected: P2241

$ 137.00
Your price:
Add to Cart
This product is discontinued
pGEM®-5Zf(+) Vector
$ 137.00
Your price: Log in

The pGEM®-5Zf(+) Vector serves as a standard cloning vector and as a template for in vitro transcription. This vector contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for ApaI, AatII, SphI, NcoI, SacII, EcoRV, SpeI, NotI, PstI, SalI, NdeI, SacI, BstXI and NsiI.


  1. Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.

pGEM®-5Zf(+) Vector  GenBank® Accession Number X65308 and vector sequence text file.


What's in the box?

Item Part # Size

pGEM®-5Zf(+) Vector

P224A 1 × 20μg


Choose language:

Download SDSPDF (219 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


Let's find the product that meets your needs.

Talk to a Scientist