Bacterial Strain JM109(DE3)

P9801_Bacterial Strain JM109_DE3
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For High-Level Gene Expression from the T7 Promoter

  • Contains an IPTG-inducible gene for T7 RNA polymerase
  • Requires a ribosomal binding site with cloned sequence
  • Lacks blue/white cloning selection


Catalog number selected: P9801

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Bacterial Strain JM109(DE3)
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Bacterial Strain JM109(DE3), derived from JM109, contains a chromosomal copy of the gene for T7 RNA polymerase. JM109(DE3) is used for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, provided that the cloned sequence contains a ribosomal binding site. Please note: JM109(DE3) cannot be used for blue/white selection.

Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk, mk+), relA1, supE44, λ–, Δ(lac-proAB), [F´, traD36, proAB, lacIqZΔM15], lDE3.


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Item Part # Size

Bacterial Strain JM109(DE3)

P980A 1 × 500μl

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


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