NanoBRET™ Target Engagement CDK Selectivity Systems

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Profile Compound Selectivity for Cyclin-Dependent Kinases (CDKs) in Live Cells

  • Determine compound or inhibitor affinity and occupancy against up to 16 CDKs
  • Each NanoBRET™ TE CDK Selectivity System uses a simple, addition-only microplate workflow and provides a CDK Vector Panel (A or B) and a NanoBRET™ TE Kinase Assay
  • Two transfection-ready NanoBRET™ TE CDK vector panels (A and B) with 8 individual pairings of CDK-NanoLuc® fusion vector with cyclin/carrier DNA

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NanoBRET™ Target Engagement CDK Selectivity Systems
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Profile Compound Selectivity Against 16 CDKs in Live Cells

The NanoBRET™ Target Engagement (TE) CDK Selectivity Systems quantitatively measure intracellular compound affinity and occupancy against two panels of 8 CDKs each, allowing you to measure up to 16 CDKs in a single experiment. Each vector panel contains 8 individual tubes of CDK-NanoLuc® fusion vectors that are premixed with either a cyclin expression vector or carrier DNA for optimal transfections.

The CDK Selectivity Systems rely on the NanoBRET™ TE technology, a bioluminescence resonance energy transfer (BRET) method that uses the small, extremely bright NanoLuc® luciferase as the energy donor and a cell-permeable NanoBRET™ TE Tracer as the energy acceptor. The CDK Selectivity Systems use an addition-only, multiwell plate format with a single NanoBRET™ TE Kinase Assay.

How the NanoBRET™ TE CDK Selectivity Systems Work

A CDK-NanoLuc® fusion vector is co-transfected with a cyclin expression vector into cells. Within the cells, the co-expression drives association of the CDK and cyclin, creating a biased readout specific for the CDK partner protein pair. BRET is achieved by luminescent energy transfer from NanoLuc® luciferase to the cell-permeable NanoBRET™ TE Tracer that is bound to the CDK-NanoLuc® fusion protein. When the test compound is added, it competes with the tracer. This results in a dose-dependent decrease in NanoBRET™ signal, allowing intracellular affinity of the test compound to be quantitated.

Day 1 - Transfect Cells

NanoBRET™ TE CDK Selectivity System Workflow; Day 1 - Transfect Cells

Day 2 - Perform Assay

NanoBRET™ TE CDK Selectivity System Workflow; Day 2 - Perform Assay

Day 2 - Acquire Data

NanoBRET™ TE CDK Selectivity System Workflow; Day 2 - Acquire Data

Ready-to-Use Panels Simplify Cellular CDK Profiling

The NanoBRET™ TE CDK Selectivity Systems simplify routine selectivity screening with ready-to-use CDK Vector Panels. Each panel contains 8 individual tubes of CDK-NanoLuc® fusion vectors that are premixed with either a cyclin expression vector or carrier DNA for optimal transfection (see tables). Just thaw, dilute and transfect these consumable vector mixes. Panel A contains the highly studied CDKs, and Panel B contains the understudied CDKs.

NanoBRET™ TE CDK Vector Panel A Table of Vector Mixes
NanoBRET™ TE CDK Vector Panel B Table of Vector Mixes

Turn-Key Cellular CDK Profiling System

The NanoBRET™ TE CDK Selectivity Systems contain a NanoBRET™ TE CDK Vector Panel (A or B) and a single NanoBRET™ TE Intracellular Kinase Assay, K-10. The optimized assay protocol offers an easy, addition-only workflow. Begin by simply transfecting the vector panel into HEK293 cells in a multiwell plate. Following overnight incubation, add your test compounds to the assay plate and run the NanoBRET™ TE Kinase Assay, K-10, using a single, optimized concentration of NanoBRET™ Tracer K-10 for each CDK. A plate reader is used to measure BRET.

NanoBRET™ TE CDK Selectivity System Complete Workflow

Get the Data You Need

Determine intracellular affinity and compound fractional occupancy with the NanoBRET™ TE CDK Selectivity Assays.

NanoBRET™ TE CDK Selectivity System Intracellular Affinity Data Table
Graph Showing Palbociclib Compound Fractional Occupancy with NanoBRET™ TE CDK Selectivity System
Graph Showing Abemaciclib Compound Fractional Occupancy with NanoBRET™ TE CDK Selectivity System

The CDK4/6 inhibitors Palbociclib (Panel A and table) and Ribociclib (table) showed very selective engagement of CDK4/6, while Abemaciclib (Panel B and table) was CDK4/6-selective but also engaged CDK9 and the TAIRE family (table). Other inhibitors potently engaged CDK4/6 but also exhibited collateral engagement of other CDKs, including the TAIRE family. Affinities of >1µM are not reported in the table, although they are measurable as shown (Panel A).

Determine Compound Fractional Occupancy

The NanoBRET™ TE CDK Selectivity Assays can quantitate the fractional occupancy of compound for each CDK.

Table of Quantitative Fractional Occupancy Measurements for Palbociclib and Abemaciclib Using NanoBRET™ TE CDK Selectivity System

Quantitative fractional occupancy measurements for Palbociclib and Abemaciclib. Measurements were obtained by including the appropriate experimental controls, so the BRET ratio can be converted to occupancy. Fractional occupancy enables profiling compound selectivity across the CDKs at a single compound concentration. Palbociclib and Abemaciclib are CDK4/6-selective inhibitors. The occupancy data for Palbociclib at 500nM and Abemaciclib at 500nM against CDK Vector Panel A and CDK Vector Panel B are shown (Mean and Standard Deviation were calculated from 3 independent experiments).

In this study, the NanoBRET™ TE method was used to profile a collection of 46 clinically advanced CDK inhibitors and tool molecules in live cells. The results confirmed the binding of advanced clinical compounds and revealed new drug-CDK interactions. These new findings offer the potential to repurpose drugs for lesser-studied CDK family members. This paper highlights the importance of determining drug selectivity across the CDK family in live cells. The NanoBRET™ TE CDK Selectivity Systems expanded on this work, simplifying the workflow to use a single NanoBRET™ TE Assay (K-10) and ready-to-use CDK Vector Panels.

Wells, C.I. et al. (2020) Quantifying CDK inhibitor selectivity in live cells. Nat. Comm. 11, 2743.

Read the Paper Read Summary Blog
natcommspaper

Specifications

You are viewing: NP5000 Change Configuration

What's in the box?

Item Part # Size

CDK1/CCNB1 Vector Mix

NP501A 1 × 120μl

CDK2/CCNE1 Vector Mix

NP502A 1 × 120μl

CDK3/CCNE1 Vector Mix

NP503A 1 × 120μl

CDK4/CCND3 Vector Mix

NP504A 1 × 120μl

CDK5/CDK5R1 Vector Mix

NP505A 1 × 120μl

CDK6/CCDN1 Vector Mix

NP506A 1 × 120μl

CDK7/Carrier Vector Mix

NP507A 1 × 120μl

CDK9/CCNK Vector Mix

NP508A 1 × 120μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

NanoBRET™ TE Selectivity Vector Panel Limited Use Label License

BY USE OF THIS PRODUCT, USER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If user is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide user with a full refund.

User may use this product for research use only; however, no amplification or isolation of the vector components of this product and no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. User shall have no right to manufacture or have a third party manufacture the vector components within this product for any purpose. User shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. Notwithstanding the foregoing, user may use this product in provision of services to third parties in exchange for consideration; however, user may not transfer the product to third parties. No other use of this product is authorized without the prior express written consent of Promega.

In addition, user must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.

For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), user must:
(2a) use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET™-branded LARs (e.g., NanoBRET™ Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

Specifications

You are viewing: NP5050 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Transfection Carrier DNA

E488B 1 × 100μg View Product

NanoBRET™ Nano-Glo® Substrate

N157C 1 × 330μl View Product

Tracer Dilution Buffer

N219A 1 × 5ml View Product

Extracellular NanoLuc® Inhibitor

N235B 1 × 110μl View Product

NanoBRET™ Tracer K-10

N264B 1 × 300μl 400μM

LIMK1-NanoLuc® Fusion Vector

NV339A 1 × 20μg 1μg/μl

CDK1/CCNB1 Vector Mix

NP501A 1 × 120μl

CDK2/CCNE1 Vector Mix

NP502A 1 × 120μl

CDK3/CCNE1 Vector Mix

NP503A 1 × 120μl

CDK4/CCND3 Vector Mix

NP504A 1 × 120μl

CDK5/CDK5R1 Vector Mix

NP505A 1 × 120μl

CDK6/CCDN1 Vector Mix

NP506A 1 × 120μl

CDK7/Carrier Vector Mix

NP507A 1 × 120μl

CDK9/CCNK Vector Mix

NP508A 1 × 120μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

NanoBRET™ TE Selectivity Vector Panel Limited Use Label License

BY USE OF THIS PRODUCT, USER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If user is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide user with a full refund.

User may use this product for research use only; however, no amplification or isolation of the vector components of this product and no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. User shall have no right to manufacture or have a third party manufacture the vector components within this product for any purpose. User shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. Notwithstanding the foregoing, user may use this product in provision of services to third parties in exchange for consideration; however, user may not transfer the product to third parties. No other use of this product is authorized without the prior express written consent of Promega.

In addition, user must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.

For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), user must:
(2a) use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET™-branded LARs (e.g., NanoBRET™ Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

NanoBRET™ TE Tracers Limited Use Label License

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.

In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

NanoBRET™ TE LAR Limited Use Label License

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

NanoLuc® ORF TE Vector Limited Use Label License

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. No other use of this product is authorized without the prior express written consent of Promega.

In addition, researcher must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.

For uses of this product (NanoLuc® ORF Vector) for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(2a) use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity; and
(2b) use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 10,067,149 and 10,024,862, European Pat. No. 2932267, Japanese Pat. Nos. 6751294 and 7092691 and other patents and patents pending.

U.S. Pat. No. 10,513,694, European Pat. No. 3313846 and other patents.

U.S. Pat. Nos. 9,056,885 and 10,139,400, European Pat. No. 2782916 and other patents pending.

Licensed from Kazusa Genome Technologies.

Specifications

You are viewing: NP5100 Change Configuration

What's in the box?

Item Part # Size

CDK10/CCNL2 Vector Mix

NP511A 1 × 120μl

CDK14/CCNY Vector Mix

NP512A 1 × 120μl

CDK15/CCNY Vector Mix

NP513A 1 × 120μl

CDK16/CCNY Vector Mix

NP514A 1 × 120μl

CDK17/CCNY Vector Mix

NP515A 1 × 120μl

CDK18/CCNY Vector Mix

NP516A 1 × 120μl

CDKL2/Carrier Vector Mix

NP517A 1 × 120μl

CDK20/CCNH Vector Mix

NP518A 1 × 120μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

NanoBRET™ TE Selectivity Vector Panel Limited Use Label License

BY USE OF THIS PRODUCT, USER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If user is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide user with a full refund.

User may use this product for research use only; however, no amplification or isolation of the vector components of this product and no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. User shall have no right to manufacture or have a third party manufacture the vector components within this product for any purpose. User shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. Notwithstanding the foregoing, user may use this product in provision of services to third parties in exchange for consideration; however, user may not transfer the product to third parties. No other use of this product is authorized without the prior express written consent of Promega.

In addition, user must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.

For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), user must:
(2a) use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET™-branded LARs (e.g., NanoBRET™ Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

Specifications

You are viewing: NP5150 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Transfection Carrier DNA

E488B 1 × 100μg View Product

NanoBRET™ Nano-Glo® Substrate

N157C 1 × 330μl View Product

Tracer Dilution Buffer

N219A 1 × 5ml View Product

Extracellular NanoLuc® Inhibitor

N235B 1 × 110μl View Product

NanoBRET™ Tracer K-10

N264B 1 × 300μl 400μM

LIMK1-NanoLuc® Fusion Vector

NV339A 1 × 20μg 1μg/μl

CDK10/CCNL2 Vector Mix

NP511A 1 × 120μl

CDK14/CCNY Vector Mix

NP512A 1 × 120μl

CDK15/CCNY Vector Mix

NP513A 1 × 120μl

CDK16/CCNY Vector Mix

NP514A 1 × 120μl

CDK17/CCNY Vector Mix

NP515A 1 × 120μl

CDK18/CCNY Vector Mix

NP516A 1 × 120μl

CDKL2/Carrier Vector Mix

NP517A 1 × 120μl

CDK20/CCNH Vector Mix

NP518A 1 × 120μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

NanoBRET™ TE Selectivity Vector Panel Limited Use Label License

BY USE OF THIS PRODUCT, USER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If user is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide user with a full refund.

User may use this product for research use only; however, no amplification or isolation of the vector components of this product and no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. User shall have no right to manufacture or have a third party manufacture the vector components within this product for any purpose. User shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. Notwithstanding the foregoing, user may use this product in provision of services to third parties in exchange for consideration; however, user may not transfer the product to third parties. No other use of this product is authorized without the prior express written consent of Promega.

In addition, user must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.

For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), user must:
(2a) use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET™-branded LARs (e.g., NanoBRET™ Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

NanoBRET™ TE Tracers Limited Use Label License

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.

In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

NanoBRET™ TE LAR Limited Use Label License

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

NanoLuc® ORF TE Vector Limited Use Label License

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. No other use of this product is authorized without the prior express written consent of Promega.

In addition, researcher must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.

For uses of this product (NanoLuc® ORF Vector) for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(2a) use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity; and
(2b) use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 10,067,149 and 10,024,862, European Pat. No. 2932267, Japanese Pat. Nos. 6751294 and 7092691 and other patents and patents pending.

U.S. Pat. No. 10,513,694, European Pat. No. 3313846 and other patents.

U.S. Pat. Nos. 9,056,885 and 10,139,400, European Pat. No. 2782916 and other patents pending.

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