The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The LIMK1 Kinase Enzyme System contains:
- LIMK1 Kinase, 10μg (Human, recombinant; amino acids 498–796). MW: ~65kDa
- Native Swine Myelin Basic Protein (MBP) Substrate, (1mg/ml)
- 5X Reaction Buffer, 0.1M DTT
Recombinant human LIMK1 (285–638) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. LIMK1 or LIM domain kinase 1 contains a unique combination of 2 N-terminal LIM domain and a C-terminal protein kinase domain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers that can bind to DNA/RNA as well as mediating protein-protein interactions. LIMK1 is thought to be a component of an intracellular signaling pathway that may be involved in brain development especially development of nerve cells.
NCBI Database Entry
The LIMK1 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.