The PI3K-Glo™ Class I Profiling Kit contains PI3Ks (α, β, γ and δ; 5μg each), PIP2:3PS Lipid Kinase Substrate (0.25mg) and the ADP-Glo™ Kinase Assay, 1,000 assays. The lipid substrates are supplied as frozen small unilamellar vesicles containing a mixture of phosphatidylinositol (PI) or phosphoinositol-4,5-bisphosphate (PIP2) at a 1:3 ratio with phosphatidylserine (PS) as carrier lipid. The PIP2:3PS substrate is optimized for use with class I PI3Ks. The PI:3PS substrate provides a universal PI lipid kinase substrate.
This easy-to-use luminescent assay provides sensitive detection with a high signal-to-background ratios even at low % conversion of substrate. The assay is easily scalable to 1,536-well format, reducing cost per well, and making it well-suited for screening applications.