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CellTiter-Glo® 2.0 Cell Viability Assay

A Luminescent Cell Viability Assay for Fast, Easy, Everyday Use

  • ATP assay for detection of viable cells
  • Reduced preparation time and convenient repeat use over multiple days
  • Same great performance as classic CellTiter-Glo® Cell Viability Assay

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Catalog number selected: G9241

$ 122.00
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CellTiter-Glo® 2.0 Cell Viability Assay
10ml
$ 122.00
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CellTiter-Glo cell viability assay Overview Background

CellTiter-Glo® 2.0 Measures ATP, a Key Indicator of Cell Health

CellTiter-Glo® 2.0, based on the original CellTiter-Glo® Cell Viability Assay chemistry, is a single-reagent assay with improved stability for convenient storage and simpler setup. Whether you perform cell proliferation assays in a single plate or process hundreds of plates at a time, the ready-to-use reagent is designed for fast and easy everyday use. A highly sensitive ATP assay with broad linearity, it is amenable to high- throughput screening and multiplex applications.

The CellTiter-Glo® 2.0 Assay determines the number of viable cells in culture by quantifying ATP, which indicates the presence of metabolically active cells. Luminescence readout is directly proportional to the number of viable cells in culture.

CellTiter-Glo-luminescent-atp-assay

Simple Assay Setup and Enhanced Stability

A single, ready-to-use reagent and simple "add-mix-measure" protocol mean there's no need to mix components.

The CellTiter-Glo® 2.0 reagent is stable at room temperature and at 4°C, allowing fast and easy assay implementation, and eliminating the thawing and mixing required with other ATP assays. Enhanced stability also allows convenient storage.

Percent Activity Remaining CellTiter-Glo® 2.0 Reagent Stability CellTiter-Glo® "Original" Reagent Stability
>85% Activity at 22°C 7 days 12 hours
>85% Activity at 4°C 2 months 3.5 days
CellTiter-Glo 2.0 cell viability assay protocol

Assay Performance

The CellTiter-Glo® 2.0 Cell Viability Assay exhibits the same high performance as the classic CellTiter-Glo® Assay, making it suitable for use from benchtop scale to high-throughput screening.

High sensitivity and broad linearity. Luminescence measured with the CellTiter-Glo® 2.0 Assay is proportional to the number of viable Jurkat cells in culture over three orders of magnitude in 96-well, 384-well and 1536-well plates.

Multiplex With Other Cell Viability or Cytotoxicity Assays

CellTiter-Glo® 2.0 cell viability assay can be multiplexed with other assays, reducing cell culture costs and labor. Multiplexing assays in the same sample well allows complementary measures of cell health to correct for errors in culturing, plating or assay chemistry interferences.

In the example shown here, CellTox™ Green cytotoxicity assay reagent was added to bortezemib-treated K562 cells after 48 hours of exposure. Fluorescence associated with cytotoxicity was measured. The CellTiter-Glo® 2.0 assay reagent was added and luminescence measured as an indicator of cell proliferation.


Specifications

You are viewing: G9241 Change Configuration

What's in the box?

Item Part # Size

CellTiter-Glo® 2.0 Reagent

G924A 1 × 10ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

GG See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Patent Pending.

Specifications

You are viewing: G9242 Change Configuration

What's in the box?

Item Part # Size

CellTiter-Glo® 2.0 Reagent

G924B 1 × 100ml

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

GG See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Patent Pending.

Specifications

You are viewing: G9243 Change Configuration

What's in the box?

Item Part # Size

CellTiter-Glo® 2.0 Reagent

G924C 1 × 500ml

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

GG See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Patent Pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

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