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GenePrint® 10 System

B9510

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STR Analysis for Human Cell Line Authentication

  • Amplifies ANSI-0002-Recommended Loci
  • Tolerates higher DNA amounts. Better balance for aneuploid samples
  • Reduced PCR Time. Amplification in less than 1.5 hours

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Catalog number selected: B9510

$ 682.00
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GenePrint® 10 System
50 reactions
$ 682.00
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GenePrint-Hero

10-Locus STR System for Cell Line Authentication

Cell line misidentification or contamination can derail your research. Fortunately, there is an easy way to confirm the identity of human cell lines in your laboratory: short tandem repeat (STR) analysis.

To standardize STR analysis for human cell line authentication, the American Tissue Culture Collection (ATCC) Standards Development Organization Workgroup published the ASN-0002-2011, which recommends the use of at least eight STR loci (TH01, TPOX, vWA, CSF1PO, D16S539, D7S820, D13S317 and D5S818) plus Amelogenin for gender identification.

Economical, Efficient Cell Line Authentication

The GenePrint® 10 System is a 10-locus multiplex system designed to generate a multi-locus DNA profile by amplifying human genomic DNA extracted from cell pellets, cell culture enrichment media and by direct amplification from storage cards. The system includes all reagents required for successful identification and authentication of human cell lines, as well as detection of cell line contaminants; the small kit size and lower reaction volume makes GenePrint® 10 an economical choice for confirmation of cell line identity.

The GenePrint® 10 System:

  • Meets the ASN-0002-2011 Guidelines requirements with co-amplification and detection of the recommended minimum nine STR loci (including Amelogenin).
  • Improves lab efficiencies with rapid-cycling and direct-amplification from storage card samples.
  • Simplifies data analysis and comparison using loci in concordance with publicly available databases.
  • Generates optimal heterozygote balance using up to 10ng of DNA template.
  • Delivers greater consistency in detecting low-level mixtures, contaminants and genetic aberrations.

Configuration of the GenePrint® 10 System

13670MB-W
GenePrint® 10 provides co-amplification and four-color detection of ten loci, including all ASN-0002-2011 loci (TH01, TPOX, vWA, CSF1PO, D16S539, D7S820, D13S317, D5S818) plus Amelogenin and D21S11.

Ready to Use in Your Workflow

Compatible with Direct Amplification

11349ta-2-w
Amplification of DNA from the HT-29 cell line on a 1.2mm punch from an FTA® card. Amplified products were separated on an Applied Biosystems® 3130 Genetic Analyzer (3kV, 4-second injection). Inter- and intra-imbalance is expected when working with cell lines in storage cards.

Improved Balance with Large Template Input

11592ma-2-w
Amplification of 10ng of human DNA analyzed using an Applied Biosystems® 3130 Genetic Analyzer (3.0 kV, 4-second injection).

Detection Instrument Compatibility and Data Analysis

The GenePrint® 10 System is compatible with the ABI PRISM® 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. You may need to optimize protocols including the amount of template DNA, cycle number, injection conditions and loading volume for your laboratory instrumentation.

Panels and bins text files are required for automatic assignment of genotypes using the GeneMapper® and GeneMarker® software and are available for download. 

Download Panels and Bins

Protocols

Complete Protocol

Specifications

What's in the box?

Item Part # Size Concentration Available Separately

GenePrint® 10 5X Primer Pair Mix

 B934A 1 × 250μl

GenePrint® 10 5X Master Mix

 B935A 1 × 250μl

GenePrint® 10 Allelic Ladder Mix

 B937A 1 × 50μl

2800M Control DNA

 DD710A 1 × 25μl 10ng/μl View Product

Internal Lane Standard 600

 DG107A 1 × 150μl View Product

Water, Amplification Grade

 DW099A 1 × 1,250μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Resources

Articles

Citations

Software and Firmware

Frequently Used With

Spectrum Compact CE System

An affordable benchtop instrument for Sanger sequencing and fragment analysis.

CE1304, CE1307, CE1305

GenePrint® 5C Matrix Standard

Matrix fragments for spectral calibration when using the GenePrint® 24 System.

B1930

PowerPlex® Matrix Standards

DNA fragments labeled with fluorescent dyes for use with the PowerPlex® Systems.

DG4640, DG4800, DG5640, DG4850, DG4900

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