GenePrint® 10 System

10-Locus STR System for Cell Line Authentication

Cell line misidentification or contamination can derail your research. Fortunately, there is an easy way to confirm the identity of human cell lines in your laboratory: short tandem repeat (STR) analysis.

To standardize STR analysis for human cell line authentication, the American Tissue Culture Collection (ATCC) Standards Development Organization Workgroup published the ASN-0002-2011, which recommends the use of at least eight STR loci (TH01, TPOX, vWA, CSF1PO, D16S539, D7S820, D13S317 and D5S818) plus Amelogenin for gender identification.

Economical, Efficient Cell Line Authentication

The GenePrint® 10 System is a 10-locus multiplex system designed to generate a multi-locus DNA profile by amplifying human genomic DNA extracted from cell pellets, cell culture enrichment media and by direct amplification from storage cards. The system includes all reagents required for successful identification and authentication of human cell lines, as well as detection of cell line contaminants; the small kit size and lower reaction volume makes GenePrint® 10 an economical choice for confirmation of cell line identity.

The GenePrint® 10 System:

Meets the ASN-0002-2011 Guidelines requirements with co-amplification and detection of the recommended minimum nine STR loci (including Amelogenin).
Improves lab efficiencies with rapid-cycling and direct-amplification from storage card samples.
Simplifies data analysis and comparison using loci in concordance with publicly available databases.
Generates optimal heterozygote balance using up to 10ng of DNA template.
Delivers greater consistency in detecting low-level mixtures, contaminants and genetic aberrations.

Configuration of the GenePrint® 10 System

GenePrint® 10 provides co-amplification and four-color detection of ten loci, including all ASN-0002-2011 loci (TH01, TPOX, vWA, CSF1PO, D16S539, D7S820, D13S317, D5S818) plus Amelogenin and D21S11.

Ready to Use in Your Workflow

Compatible with Direct Amplification

11349ta-2-w — Amplification of DNA from the HT-29 cell line on a 1.2mm punch from an FTA® card. Amplified products were separated on an Applied Biosystems® 3130 Genetic Analyzer (3kV, 4-second injection). Inter- and intra-imbalance is expected when working with cell lines in storage cards.

Improved Balance with Large Template Input

11592ma-2-w — Amplification of 10ng of human DNA analyzed using an Applied Biosystems® 3130 Genetic Analyzer (3.0 kV, 4-second injection).

Detection Instrument Compatibility and Data Analysis

The GenePrint® 10 System is compatible with the ABI PRISM® 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. You may need to optimize protocols including the amount of template DNA, cycle number, injection conditions and loading volume for your laboratory instrumentation.

Panels and bins text files are required for automatic assignment of genotypes using the GeneMapper® and GeneMarker® software and are available for download.

Download Panels and Bins

Protocols

Complete Protocol

GenePrint® 10 System Technical Manual

PDF (2 MB) – English

What's in the box?

Item	Part #	Size	Concentration	Available Separately
GenePrint® 10 5X Primer Pair Mix	B934A	1 × 250μl
GenePrint® 10 5X Master Mix	B935A	1 × 250μl
GenePrint® 10 Allelic Ladder Mix	B937A	1 × 50μl
2800M Control DNA	DD710A	1 × 25μl	10ng/μl	View Product
Internal Lane Standard 600	DG107A	1 × 150μl		View Product
Water, Amplification Grade	DW099A	1 × 1,250μl		View Product

SDS

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Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

Australian Pat. No. 724531 and other patents and patents pending.

Allele sequences for one or more of the loci vWA, FGA, D8S1179, D21S11 and D18S51 in allelic ladder mixtures is licensed under U.S. Pat. Nos. 7,087,380 and 7,645,580, Australia Pat. No. 2003200444 and corresponding patent claims outside the US.

Resources

Articles

Citations

Acquired RAS or EGFR mutations and duration of response to EGFR blockade in colorectal cancer.
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2016 Nat. Commun.
See all citations

Software and Firmware

GenePrint 10 System Software Panels and Bin Files

Frequently Used With

PowerPlex® Matrix Standards

DNA fragments labeled with fluorescent dyes for use with the PowerPlex® Systems.

DG4640, DG4800, DG5640, DG4850, DG4900

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GenePrint® 10 System

STR Analysis for Human Cell Line Authentication

10-Locus STR System for Cell Line Authentication