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Western Blue® Stabilized Substrate for Alkaline Phosphatase

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  • Ready-to-use formulation that does not require dilution or reagent mixing
  • As sensitive as other commercially available BCIP/NBT formulations and reagents
  • Stable for one year at room temperature


Catalog number selected: S3841

$ 69.00
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Western Blue® Stabilized Substrate for Alkaline Phosphatase
$ 69.00
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Western Blue® Stabilized Substrate for Alkaline Phosphatase is a stable, ready-to-use substrate for Western blots and immunoscreening (e.g., dot blots). It is a mixture of 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) and nitro blue tetrazolium (NBT) in a proprietary stabilizing buffer. Western Blue® Substrate should be used directly and without dilution. This liquid substrate deposits a permanent dark purple stain on membrane sites bearing alkaline phosphatase. Western Blue® Substrate is as sensitive as other reagents based on the BCIP/NBT formulation.


  1. Cedeno-Laurent, F. et al. (2012) Galectin-1 triggers an immunoregulatory signature in Th cells functionally defined by IL-10 expression. J. Immunol. 188, 3127–37.
  2. Petrova, N.S. et al. (2012) Carrier-free cellular uptake and the gene-silencing activity of the lipophilic siRNAs is strongly affected by the length of the linker between siRNA and lipophilic group. Nucleic Acids Res. 40, 2330–44.
  3. To, W.S. and Midwood, K.S. (2011) Identification of novel and distinct binding sites within Tenascin-C for soluble and fibrillar fibronectin. J. Biol. Chem. 286, 14881–91.
Western blot (immunoblot) for β-actin in cytoplasmic lysate from HEK293T cells.

The blot was performed on a serial dilution of lysate; each lane contains an indicated amount of β-actin (ng). Primary antibody (monoclonal) used at 1:5,000; Anti-Mouse IgG, AP Conjugate secondary antibody (Cat.# S3721) used at 1:2,500; blot imaged with Western® Blue Stabilized Substrate for Alkaline Phosphatase (Cat.# S3841).


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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


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