CRISPR Ready-to-Use Reporter Cell Lines
CRISPR-engineered cell lines with a bioluminescent tag fused to the target of interest provide a convenient and quantifiable method for studying endogenous protein abundance. Live-cell assays using these cell lines enable the study of protein dynamics in real time.
Promega continues to expand our collection of HiBiT, NanoLuc®, and HaloTag® CRISPR knock-in cell pools and clones, including both N and C-terminal fusions to many popular drug targets. Knock-ins are performed in common workhorse cell lines and, in many cases, the cell background that is most relevant to the biology being studied. Let our experts take care of the cell engineering so you can focus on your research goals.
Cell Line Pools and Clones
We offer a large selection of pre-built and in-development CRISPR knock-in cell lines. Search the table below to find your target or cell background of interest. Cell lines in development are indicated by asterisks (*).
Search List
| Target | Tag | Terminus | Background | Clone/Pool |
|---|---|---|---|---|
| ADAR1(p150) | HiBiT | N | A549 | C |
| APEX | HiBiT | C | HEK293 LgBiT | P |
| AR | HiBiT | C | 22Rv1 | C |
| AR | HiBiT | C | LNCaP clone FGC | C |
| AR(V7) | HiBiT | C | 22Rv1 | C |
| ARAF | HiBiT | N | A549 | C |
| ARID1A | HiBiT | N | ES-2 | P |
| ARID1A | HiBiT | C | ES-2 | P |
| ARID1B | HiBiT | N | TOV-21G | P |
| ARID1B | HiBiT | C | TOV-21G | P |
| AURKA | HiBiT | C | MOLT-4 | P |
| AURKA | HiBiT | C | PC-3 | P |
| BCL10 | HiBiT | C | NCI-H1299 | C |
| BCL11A | HiBiT | C | HEK293 LgBiT | C |
| BCL11A | HiBiT | N | HEK293 LgBiT | C |
| BPTF | HiBiT | N | HEK293 | P |
| BPTF | HiBiT | C | HEK293 | P |
| BRAF | HiBiT | N | A-549 | C/P |
| BRAF (V600E) | HiBiT | N | A375 | C |
| BRD2 | HiBiT | N | HEK293 LgBiT | C |
| BRD3 | HiBiT | N | HEK293 LgBiT | C |
| BRD4 | HiBiT | N | A-549 | C |
| BRD4 | HiBiT | N | HEK293 | C |
| BRD4 | HiBiT | N | HEK293 LgBiT | C |
| BRD4 | HiBiT | N | PC-3 | P |
| BRD4 | NanoLuc | N | HEK293 | C |
| BRD4 | HiBiT-HaloTag | N | HEK293 LgBiT | C |
| BRD4 (CRBN KO) | HiBiT | N | HEK293 LgBiT | C |
| BRD4 (VHL KO) | HiBiT | N | HEK293 LgBiT | C |
| BRD7 | HiBiT | C | HEK293 LgBiT | C |
| BRD7 | HiBiT | C | HEK293 | P |
| BRD7 | HiBiT | N | HEK293 LgBiT | C/P |
| BRD9 | HiBiT | C | HEK293 | P |
| BRD9 | HiBiT | C | HEK293 LgBiT | C |
| BRD9 | HiBiT | N | HEK293 LgBiT | C/P |
| BRPF1 | HiBiT | N | HEK293 | P |
| BRPF1 | HiBiT | C | HEK293 | P |
| BTK | HiBiT | C | Ramos | C |
| CBLB | HiBiT | C | Jurkat | C/P |
| CCNE1 | HiBiT | C | HEK293 | C |
| CCNE1 | HiBiT | C | NIH:OVCAR-3 | C |
| CCNK | HiBiT | N | A549 | C |
| CDK1 | HiBiT | C | HEK293 | C |
| CDK2 | NanoLuc | C | HEK293 | C |
| CDK2 | HiBiT | C | HEK293 | C |
| CDK4 | HiBiT | C | HEK293 | P |
| CDK4 | HiBiT | N | K-562 | P |
| CDK4 | NanoLuc | N | K-562 | C |
| CDK4 | NanoLuc | N | T-47D | C |
| CDK6 | HiBiT | C | K-562 | P |
| CDK6 | NanoLuc | N | K-562 | C |
| CDK6 | HiBiT | C | HEK293 | C |
| CDK7 | HiBiT | N | HEK293 | C |
| CDK9 | HiBiT | N | HEK293 | C |
| CDK9 | HiBiT | C | HEK293 | P |
| CDK12 | HiBiT | C | A549 | C |
| CREBBP | HiBiT | C | U-2 OS | C/P |
| CREBBP | HiBiT | C | H929 | P |
| CREBBP | HiBiT | C | HEK293 | P |
| CREBBP | HiBiT | N | HEK293 | P |
| CSNK1A1 | HiBiT | C | HEK293 LgBiT | C/P |
| CSNK1A1 (CRBN KO) | HiBiT | C | HEK293 LgBiT | C |
| CTNNB1 | HiBiT | C | DLD-1 | C |
| CTNNB1 | HiBiT | N | HEK293 LgBiT | C |
| CTNNB1 | HaloTag-HiBiT | C | HEK293 LgBiT | C |
| deltaN-TP63 | HiBiT | N | BxPC-3 | C |
| DDX3X | HiBiT | N | HEK293 LgBiT | C |
| DGKA (alpha) | HiBiT | N | Jurkat | C |
| DGKB (beta) | HiBiT | N | A204 | C |
| DGKZ (zeta) | HiBiT | N | Jurkat | C |
| DPP9 | HiBiT | C | HEK293 LgBiT | C |
| EGFR | HiBiT | C | A549 LgBit | C |
| EGFR | HiBiT | N | HeLa | C |
| EP300 | HiBiT | C | H929 | P |
| EP300 | HiBiT | C | HEK293 | P |
| EP300 | HiBiT | N | HEK293 | P |
| EP300 | HiBiT | C | U-2 OS | C/P |
| ERBB3 (HER3) | NanoLuc | C | AU565 | C |
| ESR1 (ER) | HiBiT | C | MCF7 | C |
| EZH2 | HiBiT | C | HEK293 | C |
| FANCM | HiBiT | C | HEK293 LgBiT | P |
| FOXA1 | HiBiT | N | 22Rv1 | C |
| GAPDH | HiBiT | C | HEK293 | P |
| GSPT1 | HiBiT | N | HEK293 LgBiT | C/P |
| HDAC6 | HiBiT | C | HeLa LgBiT | C |
| HMOX1 | HiBiT | N | A-549 | C |
| HRI (EIF2AK1) | HiBiT | C | HEK293 | C |
| HRI (EIF2AK1) | HiBiT | C | K562 | C |
| IKZF1 (Ikaros) | HiBiT | C | Jurkat LgBiT | C/P |
| IKZF2 (Helios) | HiBiT | C | Jurkat LgBiT | P |
| IKZF2 (Helios) | HiBiT | N | Jurkat LgBiT | C/P |
| IKZF3 (Aiolos) | HiBiT | N | MM.1S | C/P |
| IL17RA | HiBiT | C | HeLa | P |
| IL2 (CD38 KO) | NanoLuc | C | Jurkat | C |
| IRAK1 | HiBiT | C | NCI-H1299 | C |
| IRAK1 | NanoLuc | N | Jurkat | C |
| IRAK1 | NanoLuc | N | THP-1 | C |
| IRAK3 | NanoLuc | N | THP-1 | C |
| IRAK4 | HiBiT | C | HeLa | C |
| IRAK4 | HiBiT | C | K-562 LgBiT | C |
| IRAK4 | HiBiT | C | NCI-H1299 | C |
| IRF5 | HiBiT | N | THP-1 | C |
| IRF7 | HiBiT | N | THP-1 | C |
| JAK1 | NanoLuc | C | HEK293 | C |
| JAK2 | NanoLuc | C | TF-1 | C |
| JAK2 (V617F) | HiBiT | C | Hel92.1.7 | C |
| JAK2 (V617F) | NanoLuc | C | Hel92.1.7 | C |
| KLK3 | HiBiT | C | 22Rv1 | C |
| KRAS (G12C) | HiBiT | N | MIA PaCa-2 | C |
| KRAS (G12D) | HiBiT | N | AsPC-1 | C |
| KRAS (G12D) | HiBiT | N | HEK293 | C |
| KRAS (G12D) | HiBiT | N | SW1990 | C |
| KRAS (G12S) | HiBiT | N | A-549 | C |
| KRAS (G12V) | HiBiT | N | SW620 | C |
| KRAS (G13D) | HiBiT | N | NCI-H647 | C |
| KRAS (WT) | HiBiT | N | NCI-H1299 | C |
| KRAS (WT) | HiBiT | N | HEK293 | C |
| MAP3K14 | HaloTag-HiBiT | C | HeLa | C |
| MAP4K1 (HPK1) | NanoLuc | N | Jurkat | C |
| MAP4K1 (HPK1) | HiBiT | N | Jurkat | C/P |
| MAP4K1 (HPK1) | HiBiT-HaloTag | N | Jurkat | C |
| MAPK14 | NanoLuc | C | HEK293 | C |
| MAPT (2N4R) | HiBiT | C | IMR-32 | C |
| MAPT | HiBiT | N | IMR-32 | C |
| MARC_1 | HaloTag-HiBiT | C | HEK293 | C |
| MET | HiBiT | C | A-549 | C |
| MET | HiBiT | C | HeLa | C |
| Myc (cMyc) | HiBiT | C | HEK293 LgBiT | C |
| MYH3 | HiBiT | C | RD | C |
| NFE2L2 (NRF2) | HiBiT | N | A-549 | C/P |
| NFE2L2 (NRF2) | HiBiT | N | NCI-H460 | C |
| NRAS (WT) | HiBiT | N | HEK293 | C |
| NRAS (Q61R) | HiBiT | N | SW1271 | C |
| NTN4 (NETRIN-4) | HiBiT | C | DLD-1 | P |
| PARP1 | HiBiT | C | HeLa | P |
| PAX8 | HiBiT | N | NIH:OVCAR-3 | P |
| PBRM1 | HiBiT | C | HEK293 LgBiT | C |
| PIK3C3 | HiBiT | N | HEK293 LgBiT | C |
| PKMYT1 | HiBiT | N | HEK293 LgBiT | P |
| PKMYT1 | HiBiT | C | HEK293 LgBiT | P |
| PLK1 | HiBiT | N | A549 | P |
| POLQ | HaloTag-HiBiT | C | DLD-1 | P |
| POLQ | NanoLuc | C | DLD-1 | C/P |
| POLQ | NanoLuc | N | DLD-1 | P |
| PRMT5 | HiBiT | N | A549 | C |
| PRMT5 | HiBiT | N | H1299 | C |
| PTPN1 | HiBiT | N | Jurkat LgBiT | C |
| PTPN2 | HiBiT | N | B16-F10 | P |
| PTPN2 | HiBiT | N | Jurkat LgBiT | C |
| PTPN2 (isoform 2) | HiBiT | C | Jurkat LgBiT | C |
| PTPN6 (SHP1) | HiBiT | C | THP-1 | C |
| PTPN22 | HiBiT | N | Jurkat | C |
| RAF1 (cRAF) | HiBiT | C | A-549 | C/P |
| RAF1 (cRAF) | HiBiT | C | Panc 08.13 | C |
| RAF1 (cRAF) | HiBiT | C | HS766T | C |
| RIPK2 | HiBiT | C | THP-1 | C |
| RUBCN | HiBiT | N | HEK293 | C |
| RUBCN | HiBiT | C | HEK293 LgBiT | C |
| SALL4 | HiBiT | N | SK-N-DZ | C |
| SALL4 | HiBiT | C | SK-N-DZ | C |
| SETDB1 | HiBiT | C | A375 | C |
| SETDB1 | HiBiT | N | A375 | C |
| SMAD2 | HiBiT-HaloTag | N | THP-1 | C |
| SMAD3 | HiBiT | N | HeLa LgBiT | C |
| SMAD3 | HiBiT-HaloTag | N | THP-1 | C |
| SMARCA2 | HiBiT | C | HeLa | C/P |
| SMARCA4 | HiBiT | C | HeLa | C/P |
| SMARCA4 | HiBiT | C | HeLa LgBiT | P |
| SPI1 | HiBiT-HaloTag | N | THP-1 | C |
| STAT3 | HiBiT | C | HeLa LgBiT | C |
| STAT3-beta | HiBiT | C | HeLa LgBiT | C |
| STAT6 | HiBiT | C | Jurkat LgBiT | C |
| SUCLA-2 | HiBiT | C | 22Rv1 | C |
| α-Synuclein (SNCA) | HiBiT | C | HeLa | C |
| TEAD2 | HiBiT | N | HEK293 | C |
| TNFRSF1A | HiBiT | C | HeLa | P |
| TREM2 | HiBiT | N | THP-1 | C |
| TREM2 | HiBiT | C | THP-1 | C |
| TREM2 | HaloTag-HiBiT | C | THP-1 | C |
| TYROBP (DAP12) | HiBiT | C | THP-1 | C |
| TYROBP (DAP12) | HaloTag-HiBiT | C | THP-1 | C |
| UBASH3A | HiBiT | C | Jurkat | P |
| USP14 | HiBiT | C | HEK293 LgBiT | C/P |
| USP14 | HiBiT | N | HEK293 LgBiT | P |
| WRN | HiBiT | C | HEK293 | C |
| WRN | HiBiT | N | HCT116 | C |
| ZC3H12A | HiBiT | C | Jurkat LgBiT | C |
| ZC3H12A | HiBiT | N | Jurkat LgBiT | C |
| ZC3H12A | HiBiT | C | HeLa LgBiT | C |
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Ordering Options
Contact us to get started with the cell lines you need or to stay updated on our growing list of available knock-in cell lines.