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CRISPR Ready-to-Use Reporter Cell Lines

CRISPR-engineered cell lines with a bioluminescent tag fused to the target of interest provide a convenient and quantifiable method for studying endogenous protein abundance. Live-cell assays using these cell lines enable the study of protein dynamics in real time.

Promega continues to expand our collection of HiBiTNanoLuc®, and HaloTag® CRISPR knock-in cell pools and clones, including both N and C-terminal fusions to many popular drug targets. Knock-ins are performed in common workhorse cell lines and, in many cases, the cell background that is most relevant to the biology being studied. Let our experts take care of the cell engineering so you can focus on your research goals.

New to CRISPR? Learn about the benefits of endogenous tagging, its various applications, and how to perform CRISPR knock-ins.

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Pools and Clones Options

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Cell Line Pools and Clones

We offer a large selection of pre-built CRISPR knock-in cell lines. The tags used in these cell lines include HiBiTNanoLuc®, and HiBiT-HaloTag® tandem tags. Cell backgrounds including LgBiT allow for live cell kinetic analysis of the HiBiT-tagged protein.

Search list for your target or cell background of interest (e.g., CREBBP, HEK293)

Target Tag Terminus Background Clone/Pool
ADAR1(p150) HiBiT N A549 C
APEX HiBiT C HEK293 (LgBiT) P
AR HiBiT C 22Rv1 C
AR HiBiT C LNCaP clone FGC C
AR(V7) HiBiT C 22Rv1 C
ARAF HiBiT N A549 C
ARID1A HiBiT N ES-2 P
ARID1A HiBiT C ES-2 P
ARID1B HiBiT N TOV-21G P
ARID1B HiBiT C TOV-21G P
AURKA HiBiT C MOLT-4 P
AURKA HiBiT C PC-3 P
BCL10 HiBiT C NCI-H1299 C
BCL11A HiBiT C HEK293 (LgBiT) C
BCL11A HiBiT N HEK293 (LgBiT) C
BCL2L1 HiBiT N A549 C
BPTF HiBiT N HEK293 P
BPTF HiBiT C HEK293 P
BRAF HiBiT N A549 C/P
BRAF(V600E) HiBiT N A375 C
BRD2 HiBiT N HEK293 (LgBiT) C
BRD3 HiBiT N HEK293 (LgBiT) C
BRD4 HiBiT N A549 C
BRD4 HiBiT N HEK293 C
BRD4 NanoLuc N HEK293 C
BRD4 HiBiT N HEK293 (LgBiT) C
BRD4 HiBiT-HaloTag N HEK293 (LgBiT) C
BRD4 HiBiT N PC-3 P
BRD4 (CRBN KO) HiBiT N HEK293 (LgBiT) C
BRD4 (VHL KO) HiBiT N HEK293 (LgBiT) C
BRD7 HiBiT C HEK293 P
BRD7 HiBiT C HEK293 (LgBiT) C
BRD7 HiBiT N HEK293 (LgBiT) C/P
BRD9 HiBiT C HEK293 P
BRD9 HiBiT C HEK293 (LgBiT) C
BRD9 HiBiT N HEK293 (LgBiT) C/P
BRPF1 HiBiT N HEK293 P
BRPF1 HiBiT C HEK293 P
BTK HiBiT C Ramos C
CBLB HiBiT C Jurkat C/P
CCNE1 HiBiT C HEK293 C
CCNE1 HiBiT C NIH:OVCAR-3 C
CCNK HiBiT N A549 C
CDK1 HiBiT C HEK293 C
CDK12 HiBiT C A549 C
CDK2 NanoLuc C HEK293 C
CDK2 HiBiT C HEK293 C
CDK4 HiBiT C HEK293 P
CDK4 HiBiT N K562 P
CDK4 NanoLuc N K562 C
CDK4 NanoLuc N T-47D C
CDK6 HiBiT C HEK293 C
CDK6 HiBiT C K562 P
CDK6 NanoLuc N K562 C
CDK7 HiBiT N HEK293 C
CDK9 HiBiT N HEK293 C
CDK9 HiBiT C HEK293 P
CREBBP HiBiT C NCI-H929 P
CREBBP HiBiT C HEK293 P
CREBBP HiBiT N HEK293 P
CREBBP HiBiT C U-2 OS C/P
CSNK1A1 HiBiT C HEK293 (LgBiT) C/P
CTNNB1 HiBiT C DLD-1 C
CTNNB1 HiBiT N HEK293 (LgBiT) C
CTNNB1 HaloTag-HiBiT C HEK293 (LgBiT) C
CTSD HiBiT N SH-SY5Y C
DDX3X HiBiT N HEK293 (LgBiT) C
deltaN-TP63 HiBiT N BxPC-3 C
DGKA (alpha) HiBiT N Jurkat C
DGKB (beta) HiBiT N A204 C
DGKZ (zeta) HiBiT N Jurkat C
DPP9 HiBiT C HEK293 (LgBiT) C
EGFR HiBiT C A549 (LgBiT) C
EGFR HiBiT N HeLa C
EP300 HiBiT C NCI-H929 P
EP300 HiBiT C HEK293 P
EP300 HiBiT N HEK293 P
EP300 HiBiT C U-2 OS C/P
ERBB3 (HER3) NanoLuc C AU565 C
ESR1 (ER) HiBiT C MCF7 C
EZH2 HiBiT C HEK293 C
FANCM HiBiT C HEK293 (LgBiT) P
FANCM HiBiT N HEK293 (LgBiT) P
FANCM HiBiT N U-2 OS C/P
FANCM HiBiT C U-2 OS C/P
FOXA1 HiBiT N 22Rv1 C
GAPDH HiBiT C HEK293 P
GSPT1 HiBiT N HEK293 (LgBiT) C/P
HDAC6 HiBiT C HeLa (LgBiT) C
HMOX1 HiBiT N A549 C
HRI (EIF2AK1) HiBiT C HEK293 C
HRI (EIF2AK1) HiBiT C K562 C
IKZF1 (Ikaros) HiBiT C Jurkat (LgBiT) C/P
IKZF2 (Helios) HiBiT C Jurkat (LgBiT) P
IKZF2 (Helios) HiBiT N Jurkat (LgBiT) C/P
IKZF3 (Aiolos) HiBiT N MM.1S C/P
IL17RA HiBiT C HeLa P
IL2 (CD38 KO) NanoLuc C Jurkat C
IL4R HiBiT C THP-1 P
IRAK1 NanoLuc N Jurkat C
IRAK1 HiBiT C NCI-H1299 C
IRAK1 NanoLuc N THP-1 C
IRAK3 NanoLuc N THP-1 C
IRAK4 HiBiT C HeLa C
IRAK4 HiBiT C K562 (LgBiT) C
IRAK4 HiBiT C NCI-H1299 C
IRF5 HiBiT N THP-1 C
IRF7 HiBiT N THP-1 C
JAK1 NanoLuc C HEK293 C
JAK2 NanoLuc C TF-1 C
JAK2(V617F) HiBiT C Hel 92.1.7 C
JAK2(V617F) NanoLuc C Hel 92.1.7 C
KLK3 HiBiT C 22Rv1 C
KRAS (G12C) HiBiT N MIA PaCa-2 C
KRAS(G12D) HiBiT N AsPC-1 C
KRAS(G12D) HiBiT N HEK293 C
KRAS(G12D) HiBiT N SW 1990 C
KRAS(G12S) HiBiT N A549 C
KRAS(G12V) HiBiT N SW620 C
KRAS(G12V) HiBiT N HEK293 C
KRAS(G13D) HiBiT N NCI-H647 C
KRAS(WT) HiBiT N HEK293 C
KRAS(WT) HiBiT N NCI-H1299 C
LIG1 HiBiT C SK-OV-3 P
MAP2K1 HiBiT N NCI-H1299 C
MAP3K14 HaloTag-HiBiT C HeLa C
MAP4K1 (HPK1) NanoLuc N Jurkat C
MAP4K1 (HPK1) HiBiT N Jurkat C/P
MAP4K1 (HPK1) HiBiT-HaloTag N Jurkat C
MAPK14 NanoLuc C HEK293 C
MAPT HiBiT N IMR-32 C
MAPT (2N4R) HiBiT C IMR-32 C
MARC1 HaloTag-HiBiT C HEK293 C
MET HiBiT C A549 C
MET HiBiT C HeLa C
Myc (cMyc) HiBiT C HEK293 (LgBiT) C
MYH3 HiBiT C RD C
NFE2L2 (NRF2) HiBiT N A549 C/P
NFE2L2 (NRF2) HiBiT N NCI-H460 C
NRAS(Q61R) HiBiT N SW 1271 C
NRAS(WT) HiBiT N HEK293 C
NTN4 (NETRIN-4) HiBiT C DLD-1 P
PARP1 HiBiT C HeLa P
PAX8 HiBiT N NIH:OVCAR-3 P
PBRM1 HiBiT C HEK293 (LgBiT) C
PIK3CA(H1047R) HiBiT N T-47D C
PIK3C3 HiBiT N HEK293 (LgBiT) C
PKMYT1 HiBiT N HEK293 (LgBiT) P
PKMYT1 HiBiT C HEK293 (LgBiT) P
PLA2R1 HiBiT N A431 C
PLK1 HiBiT N A549 P
PLK4 HiBiT C CHP-212 C
POLQ HaloTag-HiBiT C DLD-1 P
POLQ NanoLuc C DLD-1 C/P
POLQ NanoLuc N DLD-1 P
PRMT5 HiBiT N A549 C
PRMT5 HiBiT N NCI-H1299 C
PTPN1 HiBiT N Jurkat (LgBiT) C
PTPN2 HiBiT N B16-F10 P
PTPN2 HiBiT N Jurkat (LgBiT) C
PTPN2 (isoform 2) HiBiT C Jurkat (LgBiT) C
PTPN22 HiBiT N Jurkat C
PTPN6 (SHP1) HiBiT C THP-1 C
RAF1 (cRAF) HiBiT C A549 C/P
RAF1 (cRAF) HiBiT C Hs 766T C
RAF1 (cRAF) HiBiT C Panc 08.13 C
RIPK1 HiBiT N A375 C
RIPK2 HiBiT C THP-1 C
RUBCN HiBiT N HEK293 C
RUBCN HiBiT C HEK293 (LgBiT) C
SALL4 HiBiT N SK-N-DZ C
SETDB1 HiBiT C A375 C
SETDB1 HiBiT N A375 C
SMAD2 HiBiT-HaloTag N THP-1 C
SMAD3 HiBiT N HeLa (LgBiT) C
SMAD3 HiBiT-HaloTag N THP-1 C
SMARCA2 HiBiT C HeLa C/P
SMARCA4 HiBiT C HeLa C/P
SMARCA4 HiBiT C HeLa (LgBiT) P
SPI1 HiBiT-HaloTag N THP-1 C
STAT3 HiBiT C HeLa (LgBiT) C
STAT3-beta HiBiT C HeLa (LgBiT) C
STAT6 HiBiT C Jurkat P
STAT6 HiBiT C Jurkat (LgBiT) C
SUCLA2 HiBiT C 22Rv1 C
α-Synuclein (SNCA) HiBiT C HeLa C
TEAD2 HiBiT N HEK293 C
TNFRSF1A HiBiT C HeLa P
TREM2 HiBiT N THP-1 C
TREM2 HiBiT C THP-1 C
TREM2 HaloTag-HiBiT C THP-1 C
TYROBP (DAP12) HiBiT C THP-1 C
TYROBP (DAP12) HaloTag-HiBiT C THP-1 C
UBASH3A HiBiT C Jurkat P
UBASH3A HiBiT N Jurkat C
UBASH3B HiBiT C Jurkat C
USP14 HiBiT C HEK293 (LgBiT) C/P
USP14 HiBiT N HEK293 (LgBiT) P
WRN HiBiT N HCT116 C
WRN HiBiT C HEK293 C
XBP1 HiBiT N MCF7 C
XBP1s HiBiT C MCF7 C
ZC3H12A HiBiT C HeLa (LgBiT) C
ZC3H12A HiBiT C Jurkat (LgBiT) C
ZC3H12A HiBiT N Jurkat (LgBiT) C

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