Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

FluoroTect™ GreenLys in vitro Translation Labeling System Technical Bulletin

Instructions for Use of Product(s)
L5001

Literature # TB285

The FluoroTect™ GreenLys in vitro Translation Labeling System allows fluorescent labeling of in vitro translation products through the use of a modified charged lysine transfer RNA labeled with the fluorophore BODIPY®-FL. Using this system, fluorescently labeled lysine residues are incorporated into nascent proteins during translation, eliminating the requirement for labeling with [35S] methionine or other radioactive amino acids. The fluorescent lysine is added to the translation reaction as a charged epsilon-labeled fluorescent lysine-tRNA complex (FluoroTect™ GreenLys tRNA) rather than a free amino acid.
Synthesized proteins are resolved by conventional SDS-PAGE analysis. Following gel electrophoresis, detection of labeled proteins is accomplished in 2–5 minutes directly “in-gel” using a laser-based fluorescent scanner. This eliminates any requirements for protein gel manipulation associated with the use of radioactive-labeled amino acids, such as fixing/drying or overnight exposure to X-ray film. The convenience of "in-gel" detection also avoids the time-consuming electroblotting and detection steps of conventional nonisotopic detection systems.

Summary of Change
The following change was made to the 12/17 revision of this document:
Section 4.B was updated to include details on instrumentation.

Printed in USA. Revised 12/17.