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Tools for Cell Metabolism: Bioluminescent NAD(P)/NAD(P)H-Glo™ Assays

Jolanta Vidugiriene is a Senior Research Scientist in Assay Design Division of R&D, where she leads the development of a broad range of novel technologies for cell metabolism, cell viability and cytotoxicity research. Jolanta received her Ph.D from Biochemistry Department, Vilnius University, Lithuania.  Prior to joining Promega , she was a postdoctoral researcher at Boston Biomedical Research Institute and later at Laboratory of Molecular Parasitology, Rockefeller University.
  • Jolanta Vidugiriene, Ph.D.

  • Senior Research Scientist

  • Original Webinar Date: Tuesday, September 10, 2013

Measuring nicotinamide adenine dinucleotides in cells and tissues can be challenging but important to understanding cellular energy metabolism especially in cancer.

In this webinar, we will discuss the challenges associated with measuring nicotinamide adenine dinucleotides in cells and tissues. We will also introduce three new bioluminescence assays for rapid and sensitive measurement of redox defining co-factors NAD(P)/NAD(P)H. These assays utilize a novel proluciferin substrate combined with diaphorase and NAD- or NADP-specific cycling enzymes allowing measurement of reduced (NADH/NADPH), non-phosphorylated (NAD/NADH) or phosphorylated (NADP/NADPH) forms of these dinucleotides. By using these assays, the role of nicotinamide adenine dinucleotides in cellular energy metabolism and cell signaling can be elucidated more rapidly and with higher precision. Read additional information below.

- Additional Information

Cancer is a disease of uncontrolled cell growth that requires cancer cells to alter metabolic pathways to survive and proliferate.  The principal mechanisms underlying this metabolic reprograming by oncogenes and tumor suppressors is still poorly understood. Nicotinamide adenine dinucleotides (NAD+, NADH, NADP+ and NADPH) are fundamental co-factors of cellular energy metabolism. These dinucleotides are essential for macromolecule biosynthesis and the maintenance of the cellular redox potential.  In addition NAD-dependent signaling pathways (e.g., mono- and poly- ADP ribosylation, protein deacetylation) are involved in regulating other processes linked to cancer development, including epigenetic regulation, cell cycle progression, DNA repair, and circadian rhythm. The central role of nicotinamide adenine dinucleotides in cellular energy metabolism and signaling makes them important target-independent nodes that link the metabolic state of cells with energy homeostasis and gene regulation. Rapid, easy-to-use assays for measuring these dinucleotides would provide a convenient tool for investigating their role in these processes.