The interaction of bromodomain proteins with histones is of current high interest for drug targeting, as modulation of this interaction has been implicated in disease. The NanoBRET™ assay in conjunction with the GloMax® Discover instrument can be used to monitor intracellular and real-time inhibition of binding to chromatin after treatment of cells with inhibitors.
This webinar will explain the NanoBRET™ Assay that is brighter and more sensitive than the standard BRET assay. This improved system uses NanoLuc® Luciferase as the donor and HaloTag® Fusion protein:HaloTag® NanoBRET™ 618 Ligand as the acceptor. Most BRET assays are limited to in vitro assays due to the limitations of the donor and acceptor. The NanoBRET™ System allows:
- Improved signal:background ratio and dynamic range over standard BRET
- Measurement of interactions in living cells
- Use of full-length proteins rather than protein domains
- Monitoring of interacting pairs closer to physiologically relevant protein levels
Senior Research Scientist
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