Maintaining Scientific Integrity Through Cell Line Authentication
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Gabriela is a Global Strategic Marketing Manager in the Genomics area in the Life Sciences Research Business Unit. Gabriela's career spans over 20 years in the laboratory and in product development, strategy and implementation related to nucleic acid amplification, expression analysis and genotyping.
Martin Ensenberger is a Senior Research Scientist in the Nucleic Acid Technology Group. Marty received his Ph.D in Molecular and Cellular Pharmacology from the University of Wisconsin in 2005. He began work at Promega as a post-doctoral fellow on a NASA funded project. The focus of his early work was to identify and multiplex novel biomarkers to asses DNA damage following exposure to space radiation. In 2007 he transitioned to the Genetic Identity research and development team as a senior research scientist. He has been a contributing or lead author on numerous peer-reviewed developmental validation manuscripts to help characterize and support many of the products launched since that time.
Gabriela Saldanha & Martin Ensenberger, PhD
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- Original Webinar Date: Tuesday, January 26, 2016
Human cell line misidentification is a common problem in the research community that can have significant negative consequences. As of January 2016, applicants for grants will be required to authenticate key biological resources, including cell lines. This webinar will provide a brief history of cell line contamination and outline a simple and inexpensive method for authenticating human cell lines. Please read below for more detailed information.
Cell culture has an important role in the study of biology and medicine, but an increasing amount of evidence shows cell lines are frequently misidentified or contaminated. Human cell line misidentification is a common, costly problem in the research community. It typically occurs as a result of contamination in a laboratory that is manipulating several different cell lines, or due to labeling errors.Consequences include wasted time and publication of erroneous data. Many journals now recommend or require authentication of cell line identity before accepting manuscripts for publication. The NIH announced that as of January 2016, applicants for grants will be required to authenticate key biological resources, including cells. Short Tandem repeat (STR) genotyping analysis is a proven technology for uniquely identifying human cell lines and is the recommended method for indentification of human tissue culture cell lines by the ATCC Standards Development Organization (ASN-0002; Authentication of Human cell Lines: Standardization of STR Profiling). Specific Topics in this webinar will include STR kit considerations, profile analysis, and database searching and matching.