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Guidelines for Developing Robust and Reliable PCR Assays

  • PCR component influence on PCR amplification
  • Methodologies for analyzing qPCR performance
  • Strategies for simplifying the qPCR assay optimization


A robust and reliable qPCR assay is critical for a variety of experimental workflows. Setting up a qPCR assay is simple in principle, but optimization can be time consuming and requires careful experimental design. In this webinar, we discussed guidelines for streamlining qPCR assay optimization.



Leta Steffen, PhD
Applications Scientist
Promega Corporation

Leta is an Applications Scientist at Promega Corporation based in Madison, WI. She received a B.A. in Biology and Physics from Lawrence University and a Ph.D. in Genetics from Harvard University. As a graduate student, Leta mapped novel genetic mutations causing muscular dystrophy, using zebrafish as a model organism. Leta has been with Promega for 8 years, focusing on nucleic acid amplification technologies.

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