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Guidelines for Developing Robust and Reliable PCR Assays

  • PCR component influence on PCR amplification
  • Methodologies for analyzing qPCR performance
  • Strategies for simplifying the qPCR assay optimization

Summary

A robust and reliable qPCR assay is critical for a variety of experimental workflows. Setting up a qPCR assay is simple in principle, but optimization can be time consuming and requires careful experimental design. In this webinar, we discussed guidelines for streamlining qPCR assay optimization.


Speaker

leta-steffen-125x125

Leta Steffen, PhD
Applications Scientist
Promega Corporation

Leta is an Applications Scientist at Promega Corporation based in Madison, WI. She received a B.A. in Biology and Physics from Lawrence University and a Ph.D. in Genetics from Harvard University. As a graduate student, Leta mapped novel genetic mutations causing muscular dystrophy, using zebrafish as a model organism. Leta has been with Promega for 8 years, focusing on nucleic acid amplification technologies.

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