Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

GTPase Assays: New Tools to the Rescue

  • GTPase signaling overview
  • GTPase families and involvement in disease
  • A new approach to measure GTPase activity
  • Data measuring the effects of GAP and GEF proteins on GTPases


GTPase proteins are key cell signaling nodes regulating diverse cellular functions. Clinical manifestations associated with deregulation of GTPases, such as seen in XXXX and YYYY, illustrate the importance of this class of hydrolase enzymes. In fact mutations in Ras GTPases are associated with one-third of all human cancers.. However, measuring the activity of these important proteins is difficult. GTPases have a very high affinity for guanine nucleotides and are slow acting enzymes, two factors that make these enzymes difficult to measure. Adding to the complexity is the need for associated proteins to help facilitate GTP hydrolysis and nucleotide exchange.



Subhanjan Mondal, PhD
Senior Research Scientist
Promega Corporation

Subhanjan Mondal is a Senior Research Scientist at Promega. He has been with Promega since 2012. Prior to joining Promega he was a Postdoctoral Fellow at Children’s Hospital Boston/Harvard Medical School. Subhanjan received his Ph.D. in Biochemistry and Cell Biology from the University of Cologne, Germany. His graduate work was in fact on GTPases and he wished that convenient assays for GTPases like the one he developed existed back then.

We love to talk science.

Sign up to receive monthly invitations to our webinars.

Sign Up