6 x 5 Ways to Ensure Your LC/MS is Healthy
Learn about a novel reference standard that will in one run:
- Interrogate LC performance through six peptides of varying hydrophobicity
- Examine MS performance through five isotopologues of each peptide
Summary
This webinar introduces the 6 x 5 LC-MS/MS Peptide Reference Mixture as a standard for performance assessment of both the LC and MS portions of your workflow. Also featured is a free software package for historical analysis of instrument performance. Monitoring the performance of your LC/MS instrument is paramount to insuring your get quality consistent data for your studies. Many labs must perform separate work to monitor the LC and MS aspects and these standards can vary from lab to lab.
Data generated by scientific instruments and decisions based on that data depend of the instruments performing consistently. Users monitor and record parameters such as LC retention time, peak width and height while also monitoring MS parameters including accuracy, resolution, signal to noise, sensitivity (LOQ and LOD) and dynamic range. Often, no single standard can serve both purposes.
The 6 x 5 LC-MS/MS Peptide Reference Mixture is composed of six sets of five isotopologues (incorporating the heavy isotopes 13C and 15N) of the same peptide sequence. Separation of the six sets will test the LC parameters of retention time, peak width and peak height. Deconvolution of the six peaks into five isotopologues each will assess accuracy and resolution of the MS instrument. To further challenge the MS instrument, the five isotopologues are present in 10-fold differences of molar abundance to assess sensitivity and dynamic range. So, in one single run, the 6 x 5 LC-MS/MS Peptide reference Mixture will allow you to assess both LC and MS parameters. Monitoring these parameters over time can alert you to changes in your system.
Speaker
Mike Rosenblatt, PhD
R&D Group Leader
Promega Corporation
Mike is the leader of the Mass Spec Reagents group at Promega Corporation, based in Madison, WI. He received his B.S. degree from Towson University and PhD from the University of Illinois at Urbana-Champaign. After completing an NIH post-doctoral fellowship at the University of Pennsylvania, he founded the Proteomics Core facility at the Children’s Hospital of Philadelphia. Prior to joining Promega he worked with Thermo-Fisher Scientific (Pierce Products) where he was a Senior Scientist focusing on the development of reagents for the preparation of samples for biological mass spectrometry.