Notes: Poly A+ RNA was isolated from HeLa cell total RNA using the PolyATtract^® mRNA Isolation System and used for RT-PCR. (1699)

Notes: The RiboMAX™ Large Scale RNA Production System was used to make reference RNA for competitive/quantitative RT-PCR. This is an informative paper about how to perform quantitative RT-PCR and ensure the reaction is linear. The authors developed a series of equations to compensate for nonequality of amplification of different templates. (1461)

Notes: The pCI Mammalian Expression Vector was used to express the p64H1 protein in 293 cells and HT-4, a clonal neuronal cell line. Inserts in the vectors were verified for expression using the TNT^® Coupled Reticulocyte Lysate System. The proteins were translated in the presence of Canine Pancreatic Microsomal Membranes (CMMs). The pGEM^®-T Vector System was used to clone the PCR product of circle rapid amplification of cDNA ends to find the true 5'-end of the p64H1 cDNA. The Tfx™-20 Reagent was used to transfect 293 cells and HT-4 neuronal cells with the p64H1-expressing pCI-based plasmid. (1206)

Notes: The Access RT-PCR System was used to demonstrate expression of the transfected cDNAs in cells. The CellTiter 96^® Non-Radioactive Cell Proliferation Assay (MTT) was used to monitor cell survival after diphtheria toxin exposure. (0698)

Notes: The GDNF E_max ImmunoAssay System was used to quantify the level of GDNF in the conditioned media of HeLa cells transduced with a recombinant adenovirus expressing GDNF. The RQ1 DNase was used to treat RNA sample to remove genomic DNA and the AMV reverse transcriptase was used for first strand cDNA synthesis. (1605)

Notes: The pGEM^®-T Vector System was used to clone a 270bp RT-PCR product from rat spinal cord mRNA.. (2007)

Notes: This article reviews the currently available techniques for screening of DMD and BMD. For BMD, the PTT is not useful for screening for mutation detection because stop mutations are rare in BMD. (1871)

Notes: The authors used Promega's Access RT-PCR System in this study. (2220)

Notes: Tth DNAPolymerase was used to perform a specific first strand cDNA synthesis in a RT-Competitive PCR Assay. (1931)

Notes: The AMV Reverse Transcriptase, RNasin^® Ribonuclease Inhibitor and Taq DNA Polymerase were used for RT-PCR to isolate a clone of the human insulin upstream factor 1, a transcription factor. The protein was expressed in vitro with the Rabbit Reticulocyte Lysate and used for gel shift assays. Reporter studies were performed in MIN6 cells. (0762)

Notes: The Wizard^® Plus Minipreps DNA Purification Resin was used to isolate apoptotic DNA from primary cultures of cerebellar granule cells. The cells were lysed with 7M Guanidine HCl, mixed with 1ml of resin, spun down, resuspended in wash solution, collected in a minicolumn, washed and eluted in water. The DNA was treated with RNase A and treated with the nucleic acid stain, SYBR Green, for quantitation versus a DNA standard. Two hundred nanograms of the isolated DNA were analyzed by agarose gel electrophoresis and ethidium bromide staining. The RQ1 RNase-Free DNase was used to treated total RNA samples prior to RT-PCR. (1217)

Notes: Poly(A+) RNA was isolated from total RNA isolated from various Xenopus tissues. The poly(A+) RNA was used in northern blots and RT-PCR. (1188)

Notes: The RNAgents^® Total RNA Isolation System was used to isolate total RNA from mouse embryos and oocytes. The isolated RNA was used for RT-PCR analysis. (1659)

Notes: The Dual-Luciferase™ Reporter System was used to quantitate the presenilin promoter activity in Neuro2a neuroblastoma cells, mouse P19 embryonal carcinoma cells and NIH 3T3 cells. Studies were also performed in P19 cells treated with retinoic acid to acquire a neuron-like phenotype and P19 cells treated with dimethyl sulfoxide to acquire a muscle-like phenotype. The presenilin promoter functioned best in the Neuro2a and neuron-like P19 cells. 5´-RACE products from mouse brain RNA were purified with the Wizard^® PCR Preps System and cloned into the pGEM-T Vector . The cloned amplimers were sequenced and used as a template for amplification to produce truncation mutants to assess promoter activity. (1590)

Notes: The Access RT-PCR System was used to amplify both lacZ and bioA in duplex. Thirty-five nanograms of bacterial RNA was amplified for 25 cycles and the amount of lacZ message was normalized to the level of bioA. (0190)

Notes: The PolyATtract^® System 1000 was used to isolate polyA+ RNA directly from newborn mouse brains. The RNA was used in RT-PCR for detection of specific TrkB variants. Taq DNA Polymerase and the pGEM^®-T Vector System were used to produce and subclone novel TrkB variants that differ in the extracellular domain. The CellTiter 96^® Non-Radioactive Cell Proliferation Assay was performed on NIH 3T3 cells transfected with the TrkB receptors and challenged with BDNF, NT3 and NT4/5. (0615)

Notes: Poly A+ RNA was isolated from mouse female fibroblast total RNA and male ES cell total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used for RT-PCR and RNase protection assays. (1682)

Notes: The RNAgents^® Total RNA Isolation System was used to isolate total RNA from human brain superior frontal gyrus. The RNA was used in RT-PCR. (1517)

Notes: Taq DNA Polymerase was used in this study. (2189)

Notes: Real-time quantitative RT-PCR was performed using the Access RT-PCR System. (1102)

Notes: Promega's Tth DNA Polymerase, pGEM^®-T Vector System and Riboprobe^® System were used in this study. (2002)

Notes: Eighteen sporadic neoplasms of ampullary origin were examined for the involvement of mutations in APC, Ras, p53 and chromosomal 5q21 allelic losses. 5q21 loss (including APC) occurred in 50% and APC mutations occurred in 17% of the ampullary tumors. 5q21 loss and mutations in APC and Ras occur in early tumor development, and p53 inactivation is associated with progression of malignancy. The researchers used PTT for APC mutation detection in the TNT® T7 Coupled Reticulocyte Lysate System with chemiluminescent labeling instead of radiolabeling of proteins. (2056)

Notes: In this study a variety of techniques were employed to screen the causative APC mutations in classical FAP cases. A combination of PTT and chemical cleavage of mismatch analysis was most effective. RT-PCR was used to amplify exons 1-14 from a lymphoblastoid cell line. 9 out of ten FAP kindreds had APC mutations that were detected with the chemical cleavage technique. PTT verified the results from the chemical cleavage analysis. The other mutation is linked to the APC gene and may be in the promoter region of the gene. (1848)

Notes: PTT was used to rapidly screen the BRCA1 gene. Transcription and translation was performed on five overlapping fragments (amplified by PCR) of the entire coding region of the BRCA1 cDNA or genomic DNA using the TNT^® T7 Coupled Reticulocyte Lysate System. (1144)

Notes: The protein truncation test was performed on PCR products from the BRCA2 gene. The PCR products were used in the TNT^® T7 Coupled Reticulocyte Lysate System to express the protein, and the protein was analyzed by SDS-PAGE. (0834)