Connell, J.P., Pammi, S., Iqbal, M.J., Huizinga, T., and Reddy, A.S.
Notes: This paper describes a quick method for analyzing plant microsatellites, also referred to as Simple Sequence Repeats (SSRs), using adaptor-ligated genomic DNA, PCR, and the Streptavidin MagneSphere® Paramagnetic Particles. Briefly, 200μg of Streptavidin MagneSphere® Paramagnetic Particles (pre-equilibrated with 6X SSC) were added to 100ng of a PCR product annealed to a biotinylated-repeat oligo. The captured fragments were washed, size-fractionated and used in PCR with AP-11 primers. (3050)