Notes: The cDNA for the short isoform of the caspase-2 mRNA was amplified and cloned directly into the pTARGET™ Mammalian Expression Vector. The protein was stably expressed in U937 human leukemic cells by selection with the antibiotic G-418.  Stable clones were identified by PCR of the genomic DNA of selected colonies with primers to the T7 promoter of the pTARGET™ Vector and the downstream primer of the caspase-2 message. Overexpression of the protein interfered with etoposide-mediated apoptosis. (2597)

Notes: The SV Total RNA Isolation System was used to isolated RNA from autopsy samples of human brain with FTDP-17 disorder. The isolated RNA was used for semi-quantitative RT-PCR (2162)

Notes: This study reports Austrian and Croatian population data for the loci contained in the PowerPlex^® 1.2 System. (2276)

Notes: Total RNA was isolated from Blue crab gills with the RNAgents^® Total RNA Isolation System. The isolated RNA was used for RT-PCR. (2562)

Notes: These researchers created alkaline phosphatase-conjugated probes that were specific for the α-proteobacterium Rhodospirillum rubrum RubisCO gene, rbcL. These probes were used in Southern and Northern blot analysis of non-transformed and transformed tobacco rubrum plants using the AttoPhos® AP Fluorescent Substrate System, a Vistra Fluorimager™ and ImageQuant™ software. The AttoPhos® Substrate was also used to visualize immunoblots of various proteins in transformed and non-transformed plants. To confirm their results, the researchers PCR-amplified choroplast DNA and ligated the products into the pGEM®-T Easy Vector for BigDye® Sequencing. (2649)

Notes: The authors of this study have developed a high throughput method for screening protein-protein interactions in 384-well plates. Using elements of the CheckMate™ Mammalian Two-Hybrid System, mouse cDNAs were linked to the GAL4 DNA binding and VP16 transcription activation domains via a linear cassette PCR strategy. The linear DNAs were mixed with the pG5luc plasmid, transfection reagent and CHO-K1 cells.  Twenty hours after transfection, activation of luciferase by GAL4 binding was assayed using the Steady-Glo^® Luciferase Assay System. Biotec and Tecan liquid handlers were used to semiautomate the system, allowing screening of 20,000 assay wells per day. (2727)

Notes: M-MLV Reverse Transcriptase and RNasin® Ribonuclease Inhibitor were used in a reaction to generate first strand cDNA.  The generated cDNA was used as a template for quantitative PCR in a Taqman® Assay.  The pGEM®-T Vector System was used to clone copies of each target gene so that standards could be generated.  The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from the S. epidermidis to generate a standard curve for quantitation of genomic DNA contamination of samples. (2291)

Notes: The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from Salmonella enterica serovars.  The isolated DNA was used as template in PCR reactions with 49 primer sets to examine the structure of the rrn operon. Some amplimers were up to 7kb in size. (2297)

Notes: The authors used TNT® T7 Quick for PCR DNA to produce protein arrays. Proteins were generated by in vitro translation directly from PCR fragments. (2138)

Notes: This is a population study reporting allele frequencies for the loci contained in the GenePrint^® PowerPlex^® 1.2 System. (2278)

Notes: This study reports that the primers used in the PowerPlex^® 16 System are reliable for typing reference samples that are used in the FBI CODIS database. The authors also report allele frequency data for the Penta D and Penta E loci. (2275)

Notes: The Wizard® Genomic DNA Purification Kit was used to purify genomic DNA from 5ml early stationary phase cultures of Borrelia burgdorferi.  Isolated genomic DNA was used in both Southern blotting and PCR. (2294)

Notes: RQ1 RNase-Free DNase was used to treat RNA samples purified from Drosophila. The DNase-treated RNA samples were then used in real-time RT-PCR to analyze gal4 and Mdh1 reporter construct transcripts driven by in vivo daughterless factor autoregulation during heat shock. (3025)

Notes: Total RNA was isolated from Mycobacterium smegmatis for Northern blot analysis and RT-PCR. Transcripts for furA and katG were amplified by RT-PCR using Promega's MMLV Reverse Transcriptase and cloned into the pGEM^®-T Easy vector. Northern blot probes for furA and katG were synthesized by in vitro transcription from the pGEM^®-T Easy vector. (2310)

Notes: The PowerPlex^® 1.2 system was used in this population study. (2268)

Notes: In this study, the amplification and typing conditions for the 13 core CODIS loci and their forensic applicability were evaluated. These loci are CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, and D21S11. The PowerPlex^® Systems were evaluated, along with other commercially available STR typing systems. (2267)

Notes: Total RNA was isolated from Rhodopseudomonas palustris using the SV Total RNA Isolation System. The Access RT-PCR System was used to determine the transcriptional organization of the badHIaliBA and badK genes, genes involved in anaerobic benzoate degradation. (2306)

Notes: These authors present the results of the 2000 and 2001 Paternity Testing Workshops, which were held to compare DNA typing results and laboratory practices between laboratories. Participating labs received a questionnaire about their processes and blood samples for DNA analysis. The results were compiled, and 91% (2000) and 86% (2001) of labs used PCR-based methods for DNA typing, and typing errors occurred in 0.3% (2000) and 0.1% (2001) of the submitted results. The PCR-based STR-typing kits used in this study included the PowerPlex® 16 System and F13A01, FESFPS, F13B, LPL Multiplex (Fluorescein). (3834)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from cultured human fibroblasts. The isolated RNA was used for RT-PCR. (2184)

Notes: The SV Total RNA Isolation System was used to isolate RNA from transiently transfected COS-7 cells bearing expression plasmid for exon 6 from a variety of patients. The isolated RNA was used for RT-PCR analysis. (2185)

Notes: To demonstrate an interaction of activated CaM KII with the postsynaptic density protein densin-180, a gst-fusion protein of densin was produced and reacted with autophosphorylated CaM KII. The glutathione matrix-bound material was eluted and immunoblotted for phospho-Thr286-CaM KII. The autophosphorylated CaM KII was pulled down on the Glutathione only when the densin-GST fusion was present. The phosphoCaM KII was detected with the Anti-ACTIVE^® CaM KII pAb. The clone of densin was generated by RT-PCR of rat forebrain total RNA with the Access RT-PCR System. (0076)

Notes: PCR product was used as a template in an in vitro transcription-translation reaction using the TNT^® T7 Coupled Reticulocyte Lysate System for a protein truncation test (PTT) assay. (1410)

Notes: The authors described a cluster of 8 genes from P. putida that is thought to be involved in benzoate metabolism: benA, benB, benC, benD, benE, benF, benK, and benR. The transcriptional start site of benA was determined using Promega's Primer Extension System-AMV Reverse Transcriptase. Total RNA used in these primer extension reactions was isolated from P. putida cells using the SV Total RNA Isolation System. The transcriptional organization of the benA, benB, and benC genes was determined by RT-PCR using the Access RT-PCR System. (2301)

Notes: The SV Total RNA Isolation System was used to isolate total RNA from rat endothelial cells. The isolated RNA was used for RT-PCR and semi-quantitative RT-PCR with cDNA generated with AMV Reverse Transcriptase. (2175)

Notes: Jurkat cells were fractionated into polysomes, and the resulting polysomes were ethanol precipitated. Total RNA was isolated from the polysomes with the SV Total RNA Isolation System. The system was also used to isolate RNA directly from Jurkat cells without fractionation. The isolated RNA was used for Northern blots, dot blots, RT-PCR and RNase protection assays. (2182)