Proc. Natl. Acad. Sci. USA
An efficient rapid system for profiling the cellular activities of molecular libraries
Melnick, J.S., Janes, J., Kim, S., Chang, J.Y., Sipes, D.G., Gunderson, D., Jarnes, L., Matzen, J.T., Garcia, M.E., Hood, T.L., Beigi, R., Xia, G., Harig, R.A., Asatryan, H., Yan, S.F., Zhou, Y., Gu, X.J., Saadat, A., Zhou, V., King, F.J., Shaw, C.M., Su, A.I., Downs, R., Gray, N.S., Schultz, P.G., Warmuth, M. and Caldwell, J.S.
Notes: The CellTiter-Glo® Luminescent Cell Viability Assay was used for a cell-based kinase assay. The authors created 35 Ba/F3 cell lines transformed with constructs encoding tyrosine kinases fused to ETV6/Tel. Fusions of tyrosine kinases to ETV6/Tel generally have constitutive activity and can confer an IL-3-independent phenotype to Ba/F3 cells. Test compounds were assayed for inhibition of kinase activity and consequent decrease in cell viability. Cell viability was assessed using the CellTiter-Glo® Assay. The assays were performed in 1536 well plates using a completely automated robotic platform. (3394)
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