Stoddard, E, Cannon, G., Ni, H., Kariko, K. Capodici, J., Malamud, D., Weissman, D.
Notes: The authors sought to identify the mediators of transmission of HIV across the mucosal barrier in humans. They noted that transmission occurs even the genital tract epithelium is seemingly not damaged. In this study they showed that gp340 expressed on primary female genital epithelial cells binds HIV-1 and enhances transmission of otherwise subinfectious amounts of HIV to infect cells, also allowing transmission to occur over longer time periods. Their study methods included plating a variety of cell types, incubating these cells with HIV, and lysing the cells with Luciferase Cell Culture Lysis Reagent, then measuring HIV-1 p24 content by ELISA. (3701)