Neumar, R.W., Xu, Y.A., Gada, H., Guttmann, R.P. and Siman R.
Notes: In this paper, researchers demonstrated that staurosporine-induced calpastatin degradation is prevented by the pan-caspase inhibitor Z-VAD-FMK. Stably transfected SH-SY5Y cells expressing calpastatin were pretreated with 50uM Z-VAD-FMK for an undisclosed amount of time before treatment with 0-0.5 µM staurosporine for 6 hours. Cell lysates were then analyzed by Western blotting to demonstrate the presence of caspase activity. (2668)