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J. Biol. Chem. 273, 26014-26025. RGSZ1, a GZ-selective RGS protein in brain: Structure, membrane association, regulation by GalphaZ, phosphorylation and relationship to a GZ GTPase-activating protein subfamily. 1998

Wang, J., Ducret, A, Tu, Y., Kozasa, T., Aebersold, R., Rose, E.

Notes: The Sequencing Grade Modified Trypsin was used to digest proteins directly on nitrocellulose by referenced methods. Briefly, nitrocellulose bands were excised, cut in 1mm squares, and incubated for 18 hours at 37°C in 15-20µl of 15mM N-ethylmorpholine, 5mM acetic acid, 1% Zwittergent 3-16 that contained 1µg of Sequencing Grade Modified Trypsin. (0201)

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J. Biol. Chem. 272, 27218-27223. cDNA cloning, tissue distribution, and identification of the catalytic triad of monoglyceride lipase. Evolutionary relationship to esterases, lysophospholipases, and haloperoxidases. 1997

Karlsson, M., Contreras, J.A., Hellman, U., Tornqvist, H., Holm, C.

Notes: Tryptic peptides, produced with Sequencing Grade Modified Trypsin, of the purified lipase were used to generate primers for RT-PCR. The largest amplimer was purified and used to screen a lambda gt11 library. The isolated 303 amino acid clone and site-specific mutants were put into the pCI-neo Mammalian Expression Vector and expressed in COS cells. The transiently expressed proteins were assayed for esterase and lipase activity. (0960)

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J. Biol. Chem. 272, 13743-13749. Isolation and characterization of a new member of the scavenger receptor superfamily, glycoprotein-340 (gp-340) as a lung surfactant protein-D binding molecule. 1997

Holmskov, U., Lawson, P., Teisner, B., Tornoe, I., Willis, A.C., Morgan, C., Koch, C., Reid, K.B.

Notes: Sequencing Grade Modified Trypsin was used to obtain sequence data from excised gel bands. (1010)

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J. Biol. Chem. 272, 14850-14859. The type II transforming growth factor-β receptor autophosphorylates not only on serine and threonine but also on tyrosine residues. 1997

Lawler, S., Feng, X.H., Chen, R.H., Maruoka, E.M., Turck, C.W., Griswold-Prenner, I., Derynck, R.

Notes: The authors used Sequencing Grade Modified Trypsin for peptide mapping. (0847)

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J. Biol. Chem. 272, 21793-21802. Topography of the photosystem I core proteins of the cyanobacterium Synechocystis sp. PCC 6803. 1997

Sun, J., Xu, Q., Chitnis, V.P., Jin, P., Chitnis, P.R.

Notes: The authors used Sequencing Grade Modified Trypsin for analysis of membrane topology of proteins (PsaA and PsaB) of photosystem I. (2192)

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J. Biol. Chem. 271, 7745-7751. Characterization of distinct nuclear and mitochondrial forms of human deoxyuridine triphosphate nucleotidohydrolase. 1996

Ladner, R.D., NcNulty, D.E., Carr, S.A., Roberts, G.D., and Caradonna, S.J.

Notes: Sequencing Grade Modified Trypsin was used to generate tryptic peptides from an SDS-PAGE gel slice. The gel slice was subjected to reduction and alkylation prior to digestion. Extensive detail of the procedure is given. (0869)

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