Cramer, J. M., Pohlmann, D., Gomez, F., Mark, L., Kornegay, B., Hall, C., Siraliev-Perez, E., Walavalkar, N. M., Sperlazza, M. J., Bilinovich, S., Prokop, J. W., Hill, A. L. and Williams, D. C. Jr.
Notes: The presence of methyl-cytosine binding domain (MBD) containing proteins and ability to remodel methylated chromatin was investigate in sponges. Specifically, the sponge MBD2 and GATAD2A protein interaction was monitored in cells using the NanoBRET™ Protein-Protein Interaction System. Multiple coiled-coil fusion constructs were tested to determine optimal signal intensity. Interestingly, while DNA methylation was observed in sponges, this interaction was much lower affinity than in vertebrate organisms. (5057)