Nuclear IGF1R Interacts with regulatory regions of chromatin to promote RNA POlymerase II recruitment and gene expression associated with advanced tumor stage.
Aleksic, T., Gray, N., Wu, X., Rieunier, G., Osher, E., Mills, J., Verrill, C., Bryant, R.J., Han, C., Hutchinson, K., Lambert, A.G., Kumar, R., Hamdy, F.C., Weyer-Czernilofsky, U., Sanderson, M.P., Bogenrieder, T., Taylor, S., Macaulay, V.M.
Notes: IGF-1R is internalized and localized to the nucleus in malignant prostate cancers. Here, the role of IGF-1R internalization is investigated using ChIP-seq. IGF-1R is shown to bind near transcription start sites of JUN and FAM21 genes and col-localized with RNA polymerase II. The proximal JUN promoter was cloned upstream of a luciferase reporter and luciferase activity was measured with the ONE-Glo EX Luciferase Assay. The presence of IDF-1R significantly increased luminescence. (5134)
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