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Citations Search

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Urology 65, 196–201. Absence of constitutional Y chromosome AZF deletions in patients with testicular germ cell tumors 2005

Lutke Holzik, M.F., Storm, K., Sijmons, R.H., D’Hollander, M., Arts, E.G.J.M., Verstraaten, M.L., Sleijfer, D.T., and Hoekstra, H.J.

Notes: In this research study, a link between infertility due to deletions in the azoospermia factors of the Y chromosome and testicular germ cell tumors (TGCTs) was investigated.  Genomic DNA isolated from peripheral blood lymphocytes of 112 Dutch patients confirmed to have TGCT was analyzed for deletions.  The deletion analysis was performed using the Y Chromosome Deletion Detection System, Version 2.0 and the previous 1.0 system plus Master Mix E.  The study finds that none of the TGCT patients had microdeletions in the AZF regions, thus suggesting a link between this type of infertility and TGCT is unlikely. (3243)

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Minerva Ginecol. 56(3), 189-96. Intracytoplasmic sperm injection. Accomplishments and qualms. 2004

Neri, Q.V., Tanaka, N., Wang, A., Katagiri, Y., Takeuchi, T., Rosenwaks, Z. and Palermo, G.D.

Notes: These authors assessed the risk of transmitting genetic defects to children after intracytoplasmic sperm injection (ICSI) for treatment of male factor infertility. Genomic DNA was isolated from blood of both parents and children using the Wizard® Genomic DNA Purification Kit. Multiplex amplification of 22 sequence tagged sites on the Y chromosome was then performed on 123 samples using the Y Chromosome Deletion Detection System, Version 1.1 and a prototype Multiplex E from Promega. (3117)

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